NICA_ARATH
ID NICA_ARATH Reviewed; 676 AA.
AC Q8GUM5; Q9LXJ9; Q9LXK0;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Nicastrin;
DE Flags: Precursor;
GN OrderedLocusNames=At3g52640/At3g52650; ORFNames=F3C22.40/F3C22.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Probable subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral membrane proteins such as Notch. {ECO:0000250}.
CC -!- SUBUNIT: Probable component of the gamma-secretase complex, a complex
CC composed of a presenilin homodimer, nicastrin, APH1 and PEN2.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GUM5-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the nicastrin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB89225.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At3g52640 and At3g52650.; Evidence={ECO:0000305};
CC Sequence=CAB89226.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At3g52640 and At3g52650.; Evidence={ECO:0000305};
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DR EMBL; AL353912; CAB89225.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL353912; CAB89226.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78974.1; -; Genomic_DNA.
DR EMBL; BT002382; AAO00742.1; -; mRNA.
DR EMBL; BT008417; AAP37776.1; -; mRNA.
DR PIR; T49017; T49017.
DR PIR; T49018; T49018.
DR RefSeq; NP_190832.3; NM_115124.5. [Q8GUM5-1]
DR AlphaFoldDB; Q8GUM5; -.
DR SMR; Q8GUM5; -.
DR BioGRID; 9748; 37.
DR STRING; 3702.AT3G52640.2; -.
DR PaxDb; Q8GUM5; -.
DR PRIDE; Q8GUM5; -.
DR ProteomicsDB; 251149; -. [Q8GUM5-1]
DR EnsemblPlants; AT3G52640.1; AT3G52640.1; AT3G52640. [Q8GUM5-1]
DR GeneID; 824430; -.
DR Gramene; AT3G52640.1; AT3G52640.1; AT3G52640. [Q8GUM5-1]
DR KEGG; ath:AT3G52640; -.
DR Araport; AT3G52640; -.
DR eggNOG; KOG2657; Eukaryota.
DR InParanoid; Q8GUM5; -.
DR OMA; CMRRNSI; -.
DR PhylomeDB; Q8GUM5; -.
DR PRO; PR:Q8GUM5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8GUM5; baseline and differential.
DR Genevisible; Q8GUM5; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR InterPro; IPR041084; Ncstrn_small.
DR InterPro; IPR008710; Nicastrin.
DR PANTHER; PTHR21092; PTHR21092; 1.
DR Pfam; PF18266; Ncstrn_small; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Notch signaling pathway;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..676
FT /note="Nicastrin"
FT /id="PRO_0000019686"
FT TOPO_DOM 29..644
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 645..665
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 666..676
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 58
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 336
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 444
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 480
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 555
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 611
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 676 AA; 73007 MW; 14632691C5A0A0A8 CRC64;
MAMGLIRLLS IAFTLVLLSI LPLHLSLADE ITSIESVPDL QKLMYVAVDG FPCVRLLNLS
GEIGCSNPGI NKVVAPIIKL KDVKDLVQPH TILVTADEME DFFTRVSTDL SFASKIGGVL
VESGSNFQQK LKGFSPDKRF PQAQFSPYEN VEYKWNSAAS SIMWRNYNFP VYLLSESGIS
AVHEILSKKK MKHGTYTSDV AEFNMVMETT KAGTHNSEAC LQEGTCLPLG GYSVWSSLPP
ISVSSSNNRK PVVLTVASMD TASFFRDKSF GADSPISGLV ALLGAVDALS RVDGISNLKK
QLVFLVLTGE TWGYLGSRRF LHELDLHSDA VAGLSNTSIE TVLEIGSVGK GLSGGINTFF
AHKTRVSSVT NMTLDALKIA QDSLASKNIK ILSADTANPG IPPSSLMAFM RKNPQTSAVV
LEDFDTNFVN KFYHSHLDDL SNINSSSVVA AASVVARTLY ILASDNKDTS NSALGSIHVN
ASFVEELLTC LLACEPGLSC NLVKDYISPT NTCPGNYAGV ILGEPSSKPY LGYVGDVSRF
LWNFLADKTS VQKGNTTSVC SKGVCSKTDE VCIKAESNKE GTCVVSTTRY VPAYSTRLKY
NDGAWTILPQ NSSDSMGMVD PVWTESNWDT LRVHVYTVQH SAYDNAVLVA GITVTTLAYI
GILAAKSIIT KALKQD
