NICA_DICDI
ID NICA_DICDI Reviewed; 659 AA.
AC Q54JT7;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Nicastrin;
DE Flags: Precursor;
GN Name=ncstn; ORFNames=DDB_G0287801;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Essential subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral membrane proteins such as Notch receptors and APP (amyloid-
CC beta precursor protein). It probably represents a stabilizing cofactor
CC required for the assembly of the gamma-secretase complex (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Component of the gamma-secretase complex, a complex
CC composed of a presenilin homodimer, nicastrin, aph1 and pen2 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nicastrin family. {ECO:0000305}.
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DR EMBL; AAFI02000104; EAL63547.2; -; Genomic_DNA.
DR RefSeq; XP_637065.2; XM_631973.2.
DR AlphaFoldDB; Q54JT7; -.
DR SMR; Q54JT7; -.
DR STRING; 44689.DDB0237950; -.
DR PaxDb; Q54JT7; -.
DR EnsemblProtists; EAL63547; EAL63547; DDB_G0287801.
DR GeneID; 8626320; -.
DR KEGG; ddi:DDB_G0287801; -.
DR dictyBase; DDB_G0287801; ncstn.
DR eggNOG; KOG2657; Eukaryota.
DR HOGENOM; CLU_029323_0_0_1; -.
DR InParanoid; Q54JT7; -.
DR OMA; CMRRNSI; -.
DR PhylomeDB; Q54JT7; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q54JT7; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR GO; GO:0070765; C:gamma-secretase complex; IMP:dictyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IMP:dictyBase.
DR GO; GO:0044351; P:macropinocytosis; IMP:dictyBase.
DR GO; GO:0006509; P:membrane protein ectodomain proteolysis; ISS:dictyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
DR GO; GO:0016485; P:protein processing; IMP:dictyBase.
DR GO; GO:0044671; P:sorocarp spore cell differentiation; IMP:dictyBase.
DR InterPro; IPR041084; Ncstrn_small.
DR InterPro; IPR008710; Nicastrin.
DR PANTHER; PTHR21092; PTHR21092; 1.
DR Pfam; PF18266; Ncstrn_small; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Notch signaling pathway; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..659
FT /note="Nicastrin"
FT /id="PRO_0000331269"
FT TOPO_DOM 23..627
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 628..648
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 649..659
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 94
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 172
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 305
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 389
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 451
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 475
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 550
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 553
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 600
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 659 AA; 74480 MW; 69F53E95D4E82F40 CRC64;
MKIKNYFIIV FIIIVLSTDV ISSQSSIEDK MYTSLNSYPC TRIMTLNGQI GCSSSHGGDS
GILYLIDSDE SYHNYFSYNQ QKDIIVVFDS NYFNKTLVLE MYSKKKMNGA LVLTDIGKTY
PYSPEDQYPI KQFGLYPDSN LNWNPNGDGF TYMNFPFPMF ALELKTSIII RNLSTINRDG
KYPAYGAELD SFMQGAINAE TCLRRGFCEP VGGQSIWSSF SEVIDQSKPI ILVMLPIDAT
AFFRDLATGT DQSGYALTVL LSMLNTLQGV DKTKWDKEVI FAMWNSERWG YVGSTNFVND
LLNFNCTSLD SNNQNSCSSP PMLDLTFEQI KFENIYAIIE FNQIGRPVNS GKKTPNKLDI
YNLVFHPNGG AGANQLMDVF SQSTQSYENS TIQFQKTTQN ELPPCSSMSF IKEINKKSAP
NFIGTLVITD HDYQYNNPYF GDEQDNSGNI NTTTSTLFDM VQVFSKSIDL LAGGNGTVKV
DDLFIREINV CLTQSITCNW VTKLMSTFPY NPIPNFYSGV YGVSPVNHIT PIETRFIFRM
ATYLTQHRTN ATNCTSDNDC DTSSSICVNK VCLYSNTHYH NAISLAFSFD NSKSSWTIVN
TSYPVFVESN WDYTTVRLFQ VGSYANEIWF LVSGLIELLL SVGIIFYIKK YLSKRYKLL
