ID   NICK_BACSU              Reviewed;         352 AA.
AC   P96635; Q797J7;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Putative DNA relaxase NicK;
GN   Name=nicK; Synonyms=ydcR; OrderedLocusNames=BSU04870;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=17693500; DOI=10.1128/jb.00932-07;
RA   Lee C.A., Grossman A.D.;
RT   "Identification of the origin of transfer (oriT) and DNA relaxase required
RT   for conjugation of the integrative and conjugative element ICEBs1 of
RT   Bacillus subtilis.";
RL   J. Bacteriol. 189:7254-7261(2007).
CC   -!- FUNCTION: Probable DNA relaxase involved in the transfer of the
CC       integrative and conjugative element ICEBs1. Required for the transfer
CC       of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT
CC       on the conjugative element and facilitates the translocation of a
CC       single strand of ICEBs1 DNA through a transmembrane conjugation pore
CC       into the recipient cell. {ECO:0000269|PubMed:17693500}.
CC   -!- MISCELLANEOUS: The oriT nick site is located within the nicK open
CC       reading frame.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB001488; BAA19324.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12294.1; -; Genomic_DNA.
DR   PIR; G69774; G69774.
DR   RefSeq; NP_388368.1; NC_000964.3.
DR   RefSeq; WP_009966621.1; NZ_JNCM01000031.1.
DR   AlphaFoldDB; P96635; -.
DR   SMR; P96635; -.
DR   STRING; 224308.BSU04870; -.
DR   PaxDb; P96635; -.
DR   PRIDE; P96635; -.
DR   EnsemblBacteria; CAB12294; CAB12294; BSU_04870.
DR   GeneID; 939922; -.
DR   KEGG; bsu:BSU04870; -.
DR   PATRIC; fig|224308.179.peg.518; -.
DR   eggNOG; COG2946; Bacteria.
DR   OMA; NITREHW; -.
DR   PhylomeDB; P96635; -.
DR   BioCyc; BSUB:BSU04870-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   InterPro; IPR040819; Rol_Rep_N.
DR   Pfam; PF02486; Rep_trans; 1.
DR   Pfam; PF18106; Rol_Rep_N; 1.
PE   3: Inferred from homology;
KW   Conjugation; DNA integration; Reference proteome.
FT   CHAIN           1..352
FT                   /note="Putative DNA relaxase NicK"
FT                   /id="PRO_0000360071"
SQ   SEQUENCE   352 AA;  40944 MW;  6455787B8E4263E2 CRC64;
     MDELKQPPHA NRGVVIVKEK NEAVESPLVS MVDYIRVSFK THDVDRIIEE VLHLSKDFMT
     EKQSGFYGYV GTYELDYIKV FYSAPDDNRG VLIEMSGQGC RQFESFLECR KKTWYDFFQD
     CMQQGGSFTR FDLAIDDKKT YFSIPELLKK AQKGECISRF RKSDFNGSFD LSDGITGGTT
     IYFGSKKSEA YLCFYEKNYE QAEKYNIPLE ELGDWNRYEL RLKNERAQVA IDALLKTKDL
     TLIAMQIINN YVRFVDADEN ITREHWKTSL FWSDFIGDVG RLPLYVKPQK DFYQKSRNWL
     RNSCAPTMKM VLEADEHLGK TDLSDMIAEA ELADKHKKML DVYMADVADM VV