ID   NICK_NOSS1              Reviewed;         351 AA.
AC   Q8YS92;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=DNA nickase {ECO:0000303|Ref.3};
DE   AltName: Full=Alr3199 protein {ECO:0000312|EMBL:BAB74898.1};
GN   OrderedLocusNames=alr3199;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ACL68399.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Nipanikar P., Rajaram H., Apte S.K.;
RT   "Cation-dependent differential activities of a novel DNA-interacting
RT   protein of Anabaena PCC7120.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:BAB74898.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [3] {ECO:0000305, ECO:0000312|EMBL:ACL68399.1}
RP   PROTEIN SEQUENCE OF 11-16; 100-107 AND 342-351, FUNCTION, INDUCTION, AND
RP   MASS SPECTROMETRY.
RA   Gokhale P.N., Singh H., Rajaram H., Apte S.K.;
RT   "Desiccation inducible protein of Anabaena 7120.";
RL   Submitted (DEC-2008) to UniProtKB.
CC   -!- FUNCTION: Acts as a DNA nickase. {ECO:0000269|Ref.3}.
CC   -!- INDUCTION: By desiccation. {ECO:0000269|Ref.3}.
CC   -!- MASS SPECTROMETRY: Mass=39709; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.3};
CC   -!- SIMILARITY: Belongs to the hemerythrin family. {ECO:0000255}.
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DR   EMBL; FJ543111; ACL68399.1; -; Genomic_DNA.
DR   EMBL; BA000019; BAB74898.1; -; Genomic_DNA.
DR   PIR; AH2205; AH2205.
DR   RefSeq; WP_010997350.1; NZ_RSCN01000001.1.
DR   AlphaFoldDB; Q8YS92; -.
DR   SMR; Q8YS92; -.
DR   STRING; 103690.17132294; -.
DR   EnsemblBacteria; BAB74898; BAB74898; BAB74898.
DR   KEGG; ana:alr3199; -.
DR   eggNOG; COG5592; Bacteria.
DR   OMA; DADQGIW; -.
DR   OrthoDB; 951806at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR012312; Hemerythrin-like.
DR   Pfam; PF01814; Hemerythrin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Iron; Metal-binding; Reference proteome;
KW   Stress response.
FT   CHAIN           1..351
FT                   /note="DNA nickase"
FT                   /id="PRO_0000395405"
FT   BINDING         241
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P02244"
FT   BINDING         245
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P02244"
FT   BINDING         245
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P02244"
FT   BINDING         303
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P02244"
SQ   SEQUENCE   351 AA;  39734 MW;  144C52A2944319C2 CRC64;
     MVSTLDDTKR NAIAEKLADA KLLQELIIEN QERFLRESTD NEISNRIRDF LEDDRKNLGI
     IETVIVQYGI QKEPRQTVRE MVDQVRQLMQ GSQLNFFEKV AQHELLKHKQ VMSGLLVHKA
     AQKVGADVLA AIGPLNTVNF ENRAHQEQLK GILEILGVRE LTGQDADQGI WGRVQDAIAA
     FSGAVGSAVT QGSDKQDMNI QDVIRMDHNK VNILFTELQQ SNDPQKIQEY FGQIYKDLTA
     HAEAEEEVLY PRVRSFYGEG DTQELYDEQS EMKRLLEQIK AISPSAPEFK DRVRQLADIV
     MDHVRQEEST LFAAIRNNLS SEQTEQWATE FKAAKSKIQQ RLGGQATGAG V