NICS_PSEPK
ID NICS_PSEPK Reviewed; 212 AA.
AC Q88FX7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=HTH-type transcriptional repressor NicS;
DE AltName: Full=Nicotinate degradation protein S;
GN Name=nicS; OrderedLocusNames=PP_3949;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
RN [2]
RP FUNCTION, PATHWAY, DISRUPTION PHENOTYPE, AND INDUCTION.
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=21450002; DOI=10.1111/j.1462-2920.2011.02471.x;
RA Jimenez J.I., Juarez J.F., Garcia J.L., Diaz E.;
RT "A finely tuned regulatory circuit of the nicotinic acid degradation
RT pathway in Pseudomonas putida.";
RL Environ. Microbiol. 13:1718-1732(2011).
CC -!- FUNCTION: Transcriptional repressor for the nicAB operon, encoding the
CC upper aerobic nicotinate degradation pathway. Acts under non-induced
CC conditions: repression of the nicAB operon becomes alleviated in
CC presence of either nicotinate or 6-hydroxynicotinate (6HNA).
CC {ECO:0000269|PubMed:21450002}.
CC -!- PATHWAY: Cofactor degradation; nicotinate degradation [regulation].
CC {ECO:0000269|PubMed:21450002}.
CC -!- INDUCTION: Expressed at a basal level under non-inducing conditions,
CC but expression increases when the cells are grown in nicotinate as sole
CC carbon source. {ECO:0000269|PubMed:21450002}.
CC -!- DISRUPTION PHENOTYPE: Growth in nicotinate or 6HNA is similar to that
CC of the wild-type strain. Mutant strain shows constitutive levels of
CC nicotinate hydroxylase activity, and these levels are similar to the
CC induced levels detected in the wild-type strain grown in nicotinate. On
CC the contrary, the NicX activity shows the same induction profile than
CC that observed in the parental strain. {ECO:0000269|PubMed:21450002}.
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DR EMBL; AE015451; AAN69543.1; -; Genomic_DNA.
DR RefSeq; NP_746079.1; NC_002947.4.
DR RefSeq; WP_010954766.1; NC_002947.4.
DR AlphaFoldDB; Q88FX7; -.
DR SMR; Q88FX7; -.
DR STRING; 160488.PP_3949; -.
DR EnsemblBacteria; AAN69543; AAN69543; PP_3949.
DR KEGG; ppu:PP_3949; -.
DR PATRIC; fig|160488.4.peg.4205; -.
DR eggNOG; COG1309; Bacteria.
DR HOGENOM; CLU_069356_1_2_6; -.
DR OMA; YDRMIYY; -.
DR PhylomeDB; Q88FX7; -.
DR BioCyc; PPUT160488:G1G01-4214-MON; -.
DR UniPathway; UPA01010; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001647; HTH_TetR.
DR InterPro; IPR041474; NicS_C.
DR InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR Pfam; PF17938; TetR_C_29; 1.
DR Pfam; PF00440; TetR_N; 1.
DR PRINTS; PR00455; HTHTETR.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF48498; SSF48498; 1.
DR PROSITE; PS50977; HTH_TETR_2; 1.
PE 2: Evidence at transcript level;
KW Aromatic hydrocarbons catabolism; DNA-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..212
FT /note="HTH-type transcriptional repressor NicS"
FT /id="PRO_0000418484"
FT DOMAIN 14..74
FT /note="HTH tetR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT DNA_BIND 37..56
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ SEQUENCE 212 AA; 24148 MW; A11F3B56C3D9175D CRC64;
MQTRKTGVRA QQADRTRDNI LKAAVKVFSK EGFTGGRIEQ ISTLAKSNDR MIYYYFGSKE
KLFISVLEHI YASFNQAEAK LRLDLADPEQ ALRELVAFIW DYYVRHPEFV TILATENLHQ
GLHARKSQNL RALSGEAVGV LRPIIEAGQA KGLFRDDICI THAYLMIASL CYFYNSNRHT
LSSFLAVDLA DKQAKADWLT FISDLALRGL RR