NICT_PSEPK
ID NICT_PSEPK Reviewed; 437 AA.
AC Q88FY6;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Putative metabolite transport protein NicT;
DE AltName: Full=Nicotinate degradation protein T;
GN Name=nicT; OrderedLocusNames=PP_3940;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=18678916; DOI=10.1073/pnas.0802273105;
RA Jimenez J.I., Canales A., Jimenez-Barbero J., Ginalski K., Rychlewski L.,
RA Garcia J.L., Diaz E.;
RT "Deciphering the genetic determinants for aerobic nicotinic acid
RT degradation: the nic cluster from Pseudomonas putida KT2440.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:11329-11334(2008).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=21450002; DOI=10.1111/j.1462-2920.2011.02471.x;
RA Jimenez J.I., Juarez J.F., Garcia J.L., Diaz E.;
RT "A finely tuned regulatory circuit of the nicotinic acid degradation
RT pathway in Pseudomonas putida.";
RL Environ. Microbiol. 13:1718-1732(2011).
CC -!- FUNCTION: Probable transporter, possibly involved in the aerobic
CC nicotinate degradation pathway. {ECO:0000305|PubMed:18678916}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Repressed by NicR in the absence of 6-hydroxynicotinate
CC (6HNA) inducer. In presence of 6HNA, repression is alleviated.
CC {ECO:0000269|PubMed:21450002}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AE015451; AAN69534.1; -; Genomic_DNA.
DR RefSeq; NP_746070.1; NC_002947.4.
DR RefSeq; WP_003251108.1; NC_002947.4.
DR AlphaFoldDB; Q88FY6; -.
DR SMR; Q88FY6; -.
DR STRING; 160488.PP_3940; -.
DR EnsemblBacteria; AAN69534; AAN69534; PP_3940.
DR KEGG; ppu:PP_3940; -.
DR PATRIC; fig|160488.4.peg.4194; -.
DR eggNOG; COG2271; Bacteria.
DR HOGENOM; CLU_001265_0_0_6; -.
DR OMA; AYAMGFW; -.
DR PhylomeDB; Q88FY6; -.
DR BioCyc; PPUT160488:G1G01-4205-MON; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Aromatic hydrocarbons catabolism; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..437
FT /note="Putative metabolite transport protein NicT"
FT /id="PRO_0000418474"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 437 AA; 47592 MW; 7C23701FA4DEFF86 CRC64;
MPIANATTVH SDIDHGTKAL YSKITWRLIP FLCFCYLAAY LDRINVGFAK LQMLEDLQFS
TAAYGLGAGL FFVGYIIFEV PSNLILQRVG AKLWIARIMI TWGLLSACTM FVTSTTQFYI
LRFLLGAAEA GFLPGVLYYL TMWYPTYRRG RIIALFMIGL PLSSVIGGPI SGWIMGHFDQ
VQGLHGWQWL FLLEAIPSVL LGILTFWALP NHFQQAKWLS ADDKAQLAAD LAADDAEGKD
SKHSFRDGFF NLKVWMLGGI DFSILLSAYA MGFWMPTFIR DAGVSDTFHI GLLTAIPSLA
ALAGMLMIGA SSDRHRERRW HIIVPFIIGA IAMASSTLFS QNLVMTVVLF AIASAAIIGA
VPVFFSLPAT FLKGTAAATG FALACSVANI AGLVSNSLMG VVTDLTGTSH AALWVFAGCL
ILSCFLVIAL PAKLVNR
