ID   NIFA_AZOCH              Reviewed;         129 AA.
AC   P56269;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Nif-specific regulatory protein;
DE   Flags: Fragment;
GN   Name=nifA;
OS   Azotobacter chroococcum mcd 1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3053983; DOI=10.1099/00221287-134-4-931;
RA   Evans D., Jones R., Woodley P., Robson R.;
RT   "Further analysis of nitrogen fixation (nif) genes in Azotobacter
RT   chroococcum: identification and expression in Klebsiella pneumoniae of
RT   nifS, nifV, nifM, and nifB genes and localization of nifE/N-, nifU-,
RT   nifA- and fixABC-like genes.";
RL   J. Gen. Microbiol. 134:931-942(1988).
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with sigma-54. {ECO:0000250}.
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DR   PIR; A60869; A60869.
DR   AlphaFoldDB; P56269; -.
DR   SMR; P56269; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   Pfam; PF02954; HTH_8; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           <1..129
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081302"
FT   DOMAIN          <1..46
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        101..129
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   129 AA;  14426 MW;  C30D30B18DF196D2 CRC64;
     EFLLTKIGRQ QGRPLTVTDS AIRLLMSHRW PGNVRDVENC LERSAIMSED GTITRDVVSL
     TGVDNESPPL AAPLPEVNLA DENLDDRERV IAALEQAGWV QAKAARLLGM TPRQIAYRIQ
     TLNIHMRKI