ID   NIFA_AZOLI              Reviewed;         624 AA.
AC   P54929;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Nif-specific regulatory protein;
GN   Name=nifA;
OS   Azospirillum lipoferum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=193;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Shigematsu T., Hidaka M., Masaki H., Uozumi T.;
RL   Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation.
CC   -!- SUBUNIT: Interacts with sigma-54.
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DR   EMBL; D13799; BAA02956.1; -; Genomic_DNA.
DR   PIR; JC5471; JC5471.
DR   AlphaFoldDB; P54929; -.
DR   SMR; P54929; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; Nif-specific_regulatory_prot.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF13185; GAF_2; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..624
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081303"
FT   DOMAIN          29..171
FT                   /note="GAF"
FT   DOMAIN          203..431
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        596..615
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          432..581
FT                   /note="Inter-domain linker"
FT   REGION          477..508
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          549..578
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          582..624
FT                   /note="C-terminal DNA-binding domain"
FT   COMPBIAS        481..504
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         231..238
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         294..303
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         445
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250|UniProtKB:P05407"
FT   BINDING         450
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250|UniProtKB:P05407"
SQ   SEQUENCE   624 AA;  67119 MW;  223A36AD444E7016 CRC64;
     MPGAMRQSTS NLELLTIYEV SKILGSSLDL QQTLREVLRA LAYQLQMHRG RVYLVGEDNV
     LRLVAANGLS NEAAAQIEFR DGEGITGRIL KTGMPAVVPN LAEEPLFLNR TGGREDLDEQ
     VASLVGVPIK AAGVVVGVLT IDRISDEGPQ GHFGSDVRFL TMVANLIGQT VRLPHVAEEP
     LRDAETFRMQ KELRPIAAPI NDVVCTSPNM LEVMAQVHRV APFKSTVLIR GESGTGKELI
     ARAIHNMSPR KDAPFIRVNC AALPESLLES ELFGHEKGAF TGAQKDHKGR FELASGGTLF
     LDEIGDISPN FQAKLLRVLQ EQEFERVGGS KTIKTDVRLI CATNLNLEEA IGHGKFRADL
     YFRINVVTIH LPPLRERRQD IGPLARHFVA KFAKDNGMTL VMEDEALEVL NRCTWPGNVR
     ELENCIERAA TQSRDGIIRT ESLSCSLNLC NSSVLFQYRT LGASVGGLAP SMGPGSVNRV
     PPGRPGVPAP ANAPKAPAMP APVPEPAGAG SAWPACASGC SAGPSPVCGA AQPAVPVPLI
     PLPLPEPSAP AAAAPAPTSV TNAAPPPAAE VPLDEPESGS LRDRLLWAME RTGWVQAKAA
     RLLGMTTRQV SYALRKYNIE IKRF