ID   NIFA_ENTAG              Reviewed;         518 AA.
AC   P54930;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Nif-specific regulatory protein;
GN   Name=nifA;
OS   Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OG   Plasmid pEA3.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX   NCBI_TaxID=549;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=333;
RX   PubMed=8544828; DOI=10.1007/bf00418032;
RA   Siddavattam D., Steibl H.D., Kreutzer R., Klingmueller W.;
RT   "Regulation of nif gene expression in Enterobacter agglomerans: nucleotide
RT   sequence of the nifLA operon and influence of temperature and ammonium on
RT   its transcription.";
RL   Mol. Gen. Genet. 249:629-636(1995).
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation.
CC   -!- SUBUNIT: Interacts with sigma-54.
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DR   EMBL; X89104; CAA61477.1; -; Genomic_DNA.
DR   EMBL; X99694; CAA68007.1; -; Genomic_DNA.
DR   PIR; S60176; S60176.
DR   AlphaFoldDB; P54930; -.
DR   SMR; P54930; -.
DR   PRIDE; P54930; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; Nif-specific_regulatory_prot.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..518
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081306"
FT   DOMAIN          35..176
FT                   /note="GAF"
FT   DOMAIN          205..432
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        490..509
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          433..475
FT                   /note="Inter-domain linker"
FT   REGION          476..518
FT                   /note="C-terminal DNA-binding domain"
FT   BINDING         232..239
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         295..304
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   518 AA;  57999 MW;  0DC4E9970010B192 CRC64;
     MTQLPTAGPV IRRFDMSAQF TALYRISVAL SQESNTARAL AAILEVLHDH AFMQYGMVCL
     FDKERNALFV ESLHGIDGER KKETRHVRYR MGEGVIGAVM SQRQALVLPR ISDDQRFLDR
     LNIYDYSLPL IGVPIPGADN QPAGVLVAQP MALHEDRLAA STRFLEMVAN LISQPLRSAT
     PPESLPAQTP VRCSVPRQFG FEQMVGKSQA MRQTMDILRQ VSKWDTTVLV RGESGTGKEL
     IANAIHYNSP RAAAPFVKFN CAALPDNLLE SELFGHEKGA FTGAIRTRKG RFELADGGTL
     FLDEIGESSA SFQAKLLRIL QEGEMERVGG DTTLKVDVRI IAATNRNLEE EVRAGNFRED
     LYYRLNVMPV SLPALRERLD DIADLAPFLV KKIALRQGRE LRISDGAVRL LMTYSWPGNV
     RELENCLERA SVMTDEGLID RDVILFNHHE SPALSVKPGL PLATDESWLD QELDERQRVI
     AALEKTGWVQ AKAARLLGMT PRQIAYRIQI MDINMHRI