ID   NIFA_KLEOX              Reviewed;         524 AA.
AC   P56266;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Nif-specific regulatory protein;
GN   Name=nifA;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NG13;
RX   PubMed=3027503; DOI=10.1007/bf00430436;
RA   Kim Y.-M., Ahn K.-J., Beppu T., Uozumi T.;
RT   "Nucleotide sequence of the nifLA operon of Klebsiella oxytoca NG13 and
RT   characterization of the gene products.";
RL   Mol. Gen. Genet. 205:253-259(1986).
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with sigma-54. {ECO:0000250}.
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DR   EMBL; D00339; BAA00245.1; -; Genomic_DNA.
DR   RefSeq; WP_004122422.1; NZ_CABGYY010000023.1.
DR   AlphaFoldDB; P56266; -.
DR   SMR; P56266; -.
DR   STRING; 571.MC52_26865; -.
DR   eggNOG; COG3604; Bacteria.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; Nif-specific_regulatory_prot.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF13185; GAF_2; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..524
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081308"
FT   DOMAIN          35..176
FT                   /note="GAF"
FT   DOMAIN          212..481
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        496..515
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..182
FT                   /note="A domain"
FT   REGION          482..524
FT                   /note="C-terminal DNA-binding domain"
FT   BINDING         240..247
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         303..312
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   524 AA;  58650 MW;  E0677A3605E6F9EE CRC64;
     MIHKSDSDTT VRRFDLSQQF TAMQRISVVL SRATEASKTL QEVLSVLHND AFMQHGMICL
     YDSQQEILSI EALQQTENQT LPGSTQIRYR PGEGLVGTVL AQGQSLVLPR VADDQRFLDR
     LSLYDYDLPF IAVPLMGPHS RPIGVLAAQP MARQEERLPA CTRFLETVAN LIAQTIRLMI
     LPTSAAQPPQ QSPRVERPRA CTSSRGFGLE NMVGKSPAMR QIMDIIRQVS RWDTTVLVRG
     ESGTGKELIA NAIHHNSPRA AAAFVKFNCA ALPDNLLESE LFGHEKGAFT GAVRQRKGRF
     ELADGGTLFL DEIGESSASF QAKLLRILQE GEMERVGGDE TLRVNVRIIA ATNRHLEEEV
     RLGHFREDLY YRLNVMPIAL PPLRERQEDI AELAHFLVRK IAHSQGRTLR ISDGAIRLLM
     EYSWPGNVRE LENCLERSAV LSESGLIDRD VILFNHRDNP PKALASSGPA EDGWLDNSLD
     ERQRLIAALE KAGWVQAKAA RLLGMTPRQV AYRIQIMDIT MPRL