ID   NIFA_RHILE              Reviewed;         519 AA.
AC   P09828;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 3.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Nif-specific regulatory protein;
GN   Name=nifA;
OS   Rhizobium leguminosarum.
OG   Plasmid sym pRL6JI.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=384;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3029674; DOI=10.1093/nar/15.1.31;
RA   Groenger P., Manian S.S., Reilaender H., O'Connell M., Priefer U.B.,
RA   Puehler A.;
RT   "Organization and partial sequence of a DNA region of the Rhizobium
RT   leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA,
RT   nifB and a novel open reading frame.";
RL   Nucleic Acids Res. 15:31-49(1987).
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation.
CC   -!- SUBUNIT: Interacts with sigma-54.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X05049; CAA28723.1; -; Genomic_DNA.
DR   PIR; C25878; C25878.
DR   AlphaFoldDB; P09828; -.
DR   SMR; P09828; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; Nif-specific_regulatory_prot.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..519
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081311"
FT   DOMAIN          177..405
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        491..510
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          406..476
FT                   /note="Inter-domain linker"
FT   REGION          477..519
FT                   /note="C-terminal DNA-binding domain"
FT   BINDING         205..212
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         268..277
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         419
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250|UniProtKB:P05407"
FT   BINDING         424
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250|UniProtKB:P05407"
SQ   SEQUENCE   519 AA;  56180 MW;  DBD1B7DB3DF69753 CRC64;
     MIKPEARLHI LYDISKELIS SFPLDNLLKA AMNALVEHLR LRDGGIVIHG SGGEPWINVR
     APIGDDVRSR SLTIEQADAI NRVIASGEKH FGKNSVVLPV KVNRKAIGAL WIDFAQKSGA
     QDESLLAMIA VLIGLTCQRD RELCSDGGSV AEEQQAGQIP KIKPKPHPTQ LDKIDWIVGE
     SPALKRVLAT TKIVAATNSA VLLRGESGTG KECFARAIHA LSIRKSKAFI KLNCAALSET
     VLESELFGHE KGAFTGALLQ RAGRFELANG GTLLLDEIGD VSPQFQAKLL RVLQEGEFER
     LGGTKTLKVD VRVICATNKN LEVAVLRGEF RADLYYRINV VPIILPPLRQ RDGDISLLAQ
     VFLEQFNNAN DRNCDFGPSA IDILSKCAFP GNVRELDNCV QRTATLASSN TITSSDFACQ
     QDQCSSALLR KADGDGIGND AMNGLNSRDT MSGGLCAHAG TPSGAAATIE AAGLTERDRL
     IKAMERAGWV QAKAARILGK TPRQVGYALR RHRIDVKKE