ID   NIFA_RHILT              Reviewed;         353 AA.
AC   P24426;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Nif-specific regulatory protein;
GN   Name=nifA;
OS   Rhizobium leguminosarum bv. trifolii.
OG   Plasmid sym pRtr843e.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ANU 843;
RX   PubMed=2552256; DOI=10.1111/j.1365-2958.1989.tb00244.x;
RA   Watson J.M., Iismaa S.E.;
RT   "The nifA gene product from Rhizobium leguminosarum biovar trifolii lacks
RT   the N-terminal domain found in other NifA proteins.";
RL   Mol. Microbiol. 3:943-955(1989).
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation.
CC   -!- SUBUNIT: Interacts with sigma-54.
CC   -!- CAUTION: This protein lacks the N-terminal domain found in other NifA
CC       proteins. {ECO:0000305}.
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DR   EMBL; X16311; CAA34377.1; -; Genomic_DNA.
DR   PIR; S05297; S05297.
DR   AlphaFoldDB; P24426; -.
DR   SMR; P24426; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; Nif-specific_regulatory_prot.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..353
FT                   /note="Nif-specific regulatory protein"
FT                   /id="PRO_0000081312"
FT   DOMAIN          12..240
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        325..344
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         103..112
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   353 AA;  38418 MW;  713BDAB4058BE0F1 CRC64;
     MPKSTVRPGA EIVGESAALK EVLEIAQIVA RSNSPVLLRG ESGTGKEFFA KLIHDSSSRH
     EKPFVKLNCA ALSAGVLESE LFGHEKGAFT GATSQKEGRF ELAHGGTLLL DEIGEISAEF
     QAKLLRVLQE GELERVGGTR TLKVNVRLVC ATNKDLETAV AAGEFRADLY YRINVVPITL
     PPLRQRDGDI PRLAQKFLQR FNRENGRSLS FAPATLDILS KCEFPGNIRE LQNCTQRTAT
     LARSDVIVPQ DLACEQGRCY SPILKKAVAE QVGKGAIHGL ARGETESMGQ PCDVGVFAAE
     TVMGQSGLIG RERLEQAMAT AGWVQAKAAR LLGRTPRQVG YSLRRHGIER KVF