ID   NIFE_BRADU              Reviewed;         547 AA.
AC   P26506; Q9ANN3;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE;
GN   Name=nifE; OrderedLocusNames=blr1745;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=2266945; DOI=10.1007/bf00262436;
RA   Aguilar O.M., Taormino J., Thoeny B., Ramseier T., Hennecke H.,
RA   Szalay A.A.;
RT   "The nifEN genes participating in FeMo cofactor biosynthesis and genes
RT   encoding dinitrogenase are part of the same operon in Bradyrhizobium
RT   species.";
RL   Mol. Gen. Genet. 224:413-420(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=11157954; DOI=10.1128/jb.183.4.1405-1412.2001;
RA   Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R.,
RA   Hennecke H.;
RT   "Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA
RT   region of the Bradyrhizobium japonicum chromosome.";
RL   J. Bacteriol. 183:1405-1412(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC       prosthetic group of nitrogenase (FeMo cofactor).
CC   -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; X56894; CAA40213.1; -; Genomic_DNA.
DR   EMBL; AF322012; AAG60731.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47010.1; -; Genomic_DNA.
DR   PIR; S12263; NIZJME.
DR   RefSeq; NP_768385.1; NC_004463.1.
DR   RefSeq; WP_011084554.1; NZ_CP011360.1.
DR   AlphaFoldDB; P26506; -.
DR   SMR; P26506; -.
DR   STRING; 224911.27349998; -.
DR   EnsemblBacteria; BAC47010; BAC47010; BAC47010.
DR   GeneID; 64067052; -.
DR   KEGG; bja:blr1745; -.
DR   PATRIC; fig|224911.44.peg.1210; -.
DR   eggNOG; COG2710; Bacteria.
DR   HOGENOM; CLU_025876_1_1_5; -.
DR   InParanoid; P26506; -.
DR   OMA; SWDNRGT; -.
DR   PhylomeDB; P26506; -.
DR   UniPathway; UPA00782; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   Gene3D; 1.10.10.1100; -; 1.
DR   InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR   InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR005973; NifE.
DR   Pfam; PF04324; Fer2_BFD; 1.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01283; nifE; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation; Reference proteome.
FT   CHAIN           1..547
FT                   /note="Nitrogenase iron-molybdenum cofactor biosynthesis
FT                   protein NifE"
FT                   /id="PRO_0000153113"
FT   CONFLICT        58
FT                   /note="T -> N (in Ref. 1; CAA40213)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        79
FT                   /note="G -> A (in Ref. 1; CAA40213)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   547 AA;  59509 MW;  F91DDCCF3984308B CRC64;
     MSSLAATVQD IFDEPGCAKN GSKSEAERRN GCTRQLQPGS AAGGCAFDGA KVALQPFTDV
     AHLVHGPIAC EGNSWDNRGA ASSGSDLWRT AFTTDLSETD IVFGGEKRLC KAIKEIIDKC
     DPPAIFVYQT CIPAMIGDDI NAVCKAASRR FSKPVIPINS PGFAGSKNLG NKLAGEALLD
     YVIGTREPDY TTPYDINLIG EYNLSGELWQ VKPVLDELGV RILCCISGDG KYREVASSHR
     ARAAMLVCSK SMINVARKME QRYGIPFFEG SFYGIQDSSE SLRQIARLLV ERGAPADLLG
     RTEAVIAREE ARAWAAIQPY KPRLEGKRAL LMTGGVKSWS VVSALQEAGL ELVGTSVKKS
     TMEDKERIKE LMGQDAHMID DMTAREMYKM LKDAEADIML SGGKSQFVAL KAAVPWVDIN
     QERCHAYMGY AGIVKLVEEI DNSLSSPMWE QLRRPAPWEA LAKAREQMQS MAAIAGDPVL
     AETARRARNI CVCNRVDLGT IEDAISVHGL RSVAAVREHT NAAGGCCQGR IEDMLMSEPS
     DMRQAAE