ID   NIFE_METBA              Reviewed;         463 AA.
AC   P51755;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE;
DE   Flags: Fragment;
GN   Name=nifE2;
OS   Methanosarcina barkeri.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2208;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43241 / DSM 1538 / 227;
RX   PubMed=8550408; DOI=10.1128/jb.178.1.143-148.1996;
RA   Chien Y.-T., Zinder S.H.;
RT   "Cloning, functional organization, transcript studies, and phylogenetic
RT   analysis of the complete nitrogenase structural genes (nifHDK2) and
RT   associated genes in the archaeon Methanosarcina barkeri 227.";
RL   J. Bacteriol. 178:143-148(1996).
CC   -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC       prosthetic group of nitrogenase (FeMo cofactor).
CC   -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; U32665; AAA96015.1; -; Genomic_DNA.
DR   AlphaFoldDB; P51755; -.
DR   SMR; P51755; -.
DR   UniPathway; UPA00782; -.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR005973; NifE.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01283; nifE; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation.
FT   CHAIN           1..>463
FT                   /note="Nitrogenase iron-molybdenum cofactor biosynthesis
FT                   protein NifE"
FT                   /id="PRO_0000153116"
FT   NON_TER         463
SQ   SEQUENCE   463 AA;  51150 MW;  1370C5DA492E929F CRC64;
     MKEKIGIVDT LEERKPYITR KQEKGQEIPL GCDNNSLAGA ISQRACVYSG ARVVLNPVTD
     AVHLVHGPIG CAGYTWDIRG AKSSGIETNR TSFSTDMKEI DVVFGGEKKL SNAIDELVEV
     YHPPVILVYS TCIVGIIGDD LESVCKTASQ KHTIHVTPVK SEGFNGNKSD GYKAACDALK
     RLIKRPSEDE IKKKAPRVPE TIKPKINILG DFNVAGDVWL VNPLFEQMGI EVIVSMTGDS
     TPKAISRAAE ADLNLVQCSG SMTYLPKWMQ AEYGIPYLNA SFFGIEDISL ALRRTADYFG
     SEEIKKRAEQ ILETEINRIM PEISRVREKV KGKKAAIYMG GPAKALTLIK GFDELGMEVV
     IIGTQTGKKE DYEQISYSVR DGTVIVDDAN PLELAELLIR QKADLMVAGV KERFIAYKLG
     IAFCDFNHDR VVEFEGFDGF VNFAREVDAS ISSPVWKAVR QRI