NIFH1_NOSS1
ID NIFH1_NOSS1 Reviewed; 295 AA.
AC P00457;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Nitrogenase iron protein 1;
DE EC=1.18.6.1;
DE AltName: Full=Nitrogenase Fe protein 1;
DE AltName: Full=Nitrogenase component II;
DE AltName: Full=Nitrogenase reductase;
GN Name=nifH1; Synonyms=nifH; OrderedLocusNames=all1455;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16592916; DOI=10.1073/pnas.77.11.6476;
RA Mevarech M., Rice D., Haselkorn R.;
RT "Nucleotide sequence of a cyanobacterial nifH gene coding for nitrogenase
RT reductase.";
RL Proc. Natl. Acad. Sci. U.S.A. 77:6476-6480(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: The key enzymatic reactions in nitrogen fixation are
CC catalyzed by the nitrogenase complex, which has 2 components: the iron
CC protein and the molybdenum-iron protein. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster per dimer. {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- PTM: The reversible ADP-ribosylation of Arg-104 inactivates the
CC nitrogenase reductase and regulates nitrogenase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NifH/BchL/ChlL family. {ECO:0000305}.
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DR EMBL; V01482; CAA24729.1; -; Genomic_DNA.
DR EMBL; V00001; CAA23398.1; -; Genomic_DNA.
DR EMBL; J05111; AAA22008.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB73411.1; -; Genomic_DNA.
DR PIR; A00534; NIAIF.
DR PIR; AC1988; AC1988.
DR RefSeq; WP_010995626.1; NZ_RSCN01000040.1.
DR AlphaFoldDB; P00457; -.
DR SMR; P00457; -.
DR STRING; 103690.17130801; -.
DR EnsemblBacteria; BAB73411; BAB73411; BAB73411.
DR KEGG; ana:all1455; -.
DR eggNOG; COG1348; Bacteria.
DR OMA; TTMMDTL; -.
DR OrthoDB; 729012at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00533; NifH; 1.
DR InterPro; IPR030655; NifH/chlL_CS.
DR InterPro; IPR000392; NifH/frxC.
DR InterPro; IPR005977; Nitrogenase_Fe_NifH.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR42864; PTHR42864; 1.
DR Pfam; PF00142; Fer4_NifH; 1.
DR PIRSF; PIRSF000363; Nitrogenase_iron; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01287; nifH; 1.
DR PROSITE; PS00746; NIFH_FRXC_1; 1.
DR PROSITE; PS00692; NIFH_FRXC_2; 1.
DR PROSITE; PS51026; NIFH_FRXC_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ADP-ribosylation; ATP-binding; Iron; Iron-sulfur; Metal-binding;
KW Nitrogen fixation; Nucleotide-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..295
FT /note="Nitrogenase iron protein 1"
FT /id="PRO_0000139481"
FT BINDING 13..20
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 101
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250"
FT MOD_RES 104
FT /note="ADP-ribosylarginine; by dinitrogenase reductase ADP-
FT ribosyltransferase"
FT /evidence="ECO:0000250"
FT CONFLICT 251
FT /note="Missing (in Ref. 1; CAA24729/CAA23398)"
FT /evidence="ECO:0000305"
FT CONFLICT 271
FT /note="L -> K (in Ref. 1; CAA24729/CAA23398/AAA22008)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="K -> NRSCRN (in Ref. 1; CAA24729/CAA23398)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 32310 MW; 411E6C6C9E58A69E CRC64;
MTDENIRQIA FYGKGGIGKS TTSQNTLAAM AEMGQRIMIV GCDPKADSTR LMLHSKAQTT
VLHLAAERGA VEDLELHEVM LTGFRGVKCV ESGGPEPGVG CAGRGIITAI NFLEENGAYQ
DLDFVSYDVL GDVVCGGFAM PIREGKAQEI YIVTSGEMMA MYAANNIARG ILKYAHSGGV
RLGGLICNSR KVDREDELIM NLAERLNTQM IHFVPRDNIV QHAELRRMTV NEYAPDSNQG
QEYRALAKKI INNDKLTIPT PMEMDELEAL LIEYGLLDDD TKHSEIIGKP AEATK
