NIFJ_ANAVA
ID NIFJ_ANAVA Reviewed; 70 AA.
AC P0C181; Q53349;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=Pyruvate-flavodoxin oxidoreductase;
DE EC=1.2.7.-;
DE Flags: Fragment;
GN Name=nifJ;
OS Anabaena variabilis.
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Trichormus.
OX NCBI_TaxID=264691;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PCC 7119 / ATCC 29151;
RX PubMed=8352652;
RA Schmitz O., Kentemich T., Zimmer W., Hundeshagen B., Bothe H.;
RT "Identification of the nifJ gene coding for pyruvate:ferredoxin
RT oxidoreductase in dinitrogen-fixing cyanobacteria.";
RL Arch. Microbiol. 160:62-67(1993).
CC -!- FUNCTION: Oxidoreductase required for the transfer of electrons from
CC pyruvate to flavodoxin, which reduces nitrogenase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CoA + 2 H(+) + oxidized [flavodoxin] + pyruvate = acetyl-CoA +
CC CO2 + reduced [flavodoxin]; Xref=Rhea:RHEA:44140, Rhea:RHEA-
CC COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC -!- SIMILARITY: Belongs to the pyruvate:ferredoxin/flavodoxin
CC oxidoreductase family. {ECO:0000305}.
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DR AlphaFoldDB; P0C181; -.
DR SMR; P0C181; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043873; F:pyruvate-flavodoxin oxidoreductase activity; IEA:RHEA.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR InterPro; IPR029061; THDP-binding.
DR Pfam; PF01855; POR_N; 1.
DR SUPFAM; SSF52518; SSF52518; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW Nitrogen fixation; Oxidoreductase; Repeat; Transport.
FT CHAIN <1..>70
FT /note="Pyruvate-flavodoxin oxidoreductase"
FT /id="PRO_0000228718"
FT NON_TER 1
FT NON_TER 70
SQ SEQUENCE 70 AA; 7304 MW; 228AC733DB15658B CRC64;
VVYQINEVIA IYPITPSSPM AEWADAWASE GKPNIWGTVP TVVQMQSEGG VAGAVHGALQ
TGSLTTTFTA