ID   NIFJ_NOSSO              Reviewed;         404 AA.
AC   Q9APC2;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Pyruvate-flavodoxin oxidoreductase;
DE            EC=1.2.7.-;
DE   Flags: Fragment;
GN   Name=nifJ;
OS   Nostoc sp. (strain ATCC 29151 / PCC 7119) (Anabaena sp.).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=1168;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11167002; DOI=10.1111/j.1574-6968.2001.tb10504.x;
RA   Schmitz O., Gurke J., Bothe H.;
RT   "Molecular evidence for the aerobic expression of nifJ, encoding
RT   pyruvate:ferredoxin oxidoreductase, in cyanobacteria.";
RL   FEMS Microbiol. Lett. 195:97-102(2001).
CC   -!- FUNCTION: Oxidoreductase required for the transfer of electrons from
CC       pyruvate to flavodoxin, which reduces nitrogenase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=CoA + 2 H(+) + oxidized [flavodoxin] + pyruvate = acetyl-CoA +
CC         CO2 + reduced [flavodoxin]; Xref=Rhea:RHEA:44140, Rhea:RHEA-
CC         COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the pyruvate:ferredoxin/flavodoxin
CC       oxidoreductase family. {ECO:0000305}.
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DR   EMBL; AF307353; AAK14076.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9APC2; -.
DR   SMR; Q9APC2; -.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043873; F:pyruvate-flavodoxin oxidoreductase activity; IEA:RHEA.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd07034; TPP_PYR_PFOR_IOR-alpha_like; 1.
DR   Gene3D; 3.40.50.920; -; 1.
DR   InterPro; IPR033412; PFOR_II.
DR   InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR009014; Transketo_C/PFOR_II.
DR   Pfam; PF17147; PFOR_II; 1.
DR   Pfam; PF01855; POR_N; 1.
DR   SUPFAM; SSF52518; SSF52518; 1.
DR   SUPFAM; SSF52922; SSF52922; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW   Nitrogen fixation; Oxidoreductase; Repeat; Transport.
FT   CHAIN           <1..>404
FT                   /note="Pyruvate-flavodoxin oxidoreductase"
FT                   /id="PRO_0000215552"
FT   NON_TER         1
FT   NON_TER         404
SQ   SEQUENCE   404 AA;  44044 MW;  0F5EEADC392B16C7 CRC64;
     IDGNEAVAQV VYQINEVIAI YPITPSSPMA EWADAWASEG KPNIWGTVPT VVQMQSEGGV
     AGAVHGALQT GSLTTTFTAS QGLLLMIPNM YKIAGELTPT VFHIAARSLA AQALSIFGDH
     SDVMATRGTG FAMLCAASVQ EAHDFALIST RTTLESRIPF LHFFDGFRTS HEINKIELLT
     TENLQTFIPN ELVIAHRSRA FTPDNSFTRG TGQNPDVYFQ GEEGTVIYYI ACQASLKSMD
     EFAQMTGRQY QLFEYHGDST AERVIVLMGS GCETVHETVD YLNTLGEKVG VIKVRLYHPF
     DSQRFIAALP PTTRSIAVLD RTKEPGASGE PLYLDVVAAL YEAGEQLPKV VGGRYGLSSK
     EFTPGMVKAV FDNLAATIPK NHFTIGINDD VSHTSLDYDP DFNI