NIFK_CROS5
ID NIFK_CROS5 Reviewed; 511 AA.
AC O07643; A1KYD7;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase;
DE AltName: Full=Nitrogenase component I;
GN Name=nifK; OrderedLocusNames=cce_0561;
OS Crocosphaera subtropica (strain ATCC 51142 / BH68) (Cyanothece sp. (strain
OS ATCC 51142)).
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC Aphanothecaceae; Crocosphaera; Crocosphaera subtropica.
OX NCBI_TaxID=43989;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10594374; DOI=10.1016/s0304-4165(99)00196-8;
RA Colon-Lopez M.S., Tang H.-Y., Tucker D.L., Sherman L.A.;
RT "Analysis of the nifHDK operon and structure of the NifH protein from the
RT unicellular, diazotrophic cyanobacterium, Cyanothece strain sp. ATCC
RT 51142.";
RL Biochim. Biophys. Acta 1473:363-375(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=18226230; DOI=10.1186/1471-2148-8-30;
RA Kneip C., Voss C., Lockhart P.J., Maier U.G.;
RT "The cyanobacterial endosymbiont of the unicellular algae Rhopalodia gibba
RT shows reductive genome evolution.";
RL BMC Evol. Biol. 8:30-30(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51142 / BH68;
RX PubMed=18812508; DOI=10.1073/pnas.0805418105;
RA Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C.,
RA Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K.,
RA Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B.;
RT "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium
RT important in the marine nitrogen cycle.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR EMBL; AF003338; AAB61284.1; -; Genomic_DNA.
DR EMBL; AY728386; AAW56990.1; -; Genomic_DNA.
DR EMBL; CP000806; ACB49912.1; -; Genomic_DNA.
DR RefSeq; WP_009546641.1; NC_010546.1.
DR AlphaFoldDB; O07643; -.
DR SMR; O07643; -.
DR STRING; 43989.cce_0561; -.
DR EnsemblBacteria; ACB49912; ACB49912; cce_0561.
DR KEGG; cyt:cce_0561; -.
DR eggNOG; COG2710; Bacteria.
DR HOGENOM; CLU_025876_2_0_3; -.
DR OMA; GLNNMID; -.
DR OrthoDB; 397330at2; -.
DR Proteomes; UP000001203; Chromosome circular.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01286; nifK; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW Nucleotide-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..511
FT /note="Nitrogenase molybdenum-iron protein beta chain"
FT /id="PRO_0000153098"
FT BINDING 69
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 94
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 187
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
SQ SEQUENCE 511 AA; 57114 MW; EF4AA3C6F804CD54 CRC64;
MAQNVNNIKD HVDLFHQPEY QELFENKKQF EGMPTAEKVQ EVAEWTKSWE YREKNFAREA
LTVNPAKACQ PLGALLAAIG FEGTLPFVHG SQGCVAYFRT HLTRHFKEPV SAVSSSMTEN
AAVFGGLKNM VDGLQNSYAL YKPKMIAVCT TCMAEVIGDD LGAFLGNARQ DGVIPDDLPV
PFAHTPSFVG SHITGYDSMM KSILSTLTEG KKKETTNGKI NFIAGFETYI GNVRAIKNII
SAFDLEGTLL SDTEMYLDSP NLGEFKMYHE GTSLEDAADS INAEATVTLQ TYTTPKTREY
IEKKWGQKTY TYRPWGVKGT DEFLMGLSEL TGKPIPKEFE IARGRAVDAM TDTQAWVHGK
RAAVYGDPDL VMGLLQFMLE MGIEPVHVLV NNSTKEFEEE AKALLAASPY GQQATVWGGK
DLWHMRSLLF TEPVDFLVGN SYAKYLQRDT KTPLIRIGYP IFDRHHLHRY STIGYEGAIN
LLNWIANGLM DELDRKTDTP SVTDISFDLV R
