ID   NIFK_HERSE              Reviewed;         356 AA.
AC   P77875;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Nitrogenase molybdenum-iron protein beta chain;
DE            EC=1.18.6.1;
DE   AltName: Full=Dinitrogenase;
DE   AltName: Full=Nitrogenase component I;
DE   Flags: Fragment;
GN   Name=nifK;
OS   Herbaspirillum seropedicae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Oxalobacteraceae; Herbaspirillum.
OX   NCBI_TaxID=964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Z78 / ATCC 35893 / DSM 6446 / LMG 6514;
RX   PubMed=9222418;
RA   Machado I.M.P., Yates M.G., Machado H.B., Souza E.M., Pedrosa F.O.;
RT   "Cloning and sequencing of the nitrogenase structural genes nifHDK of
RT   Herbaspirillum seropedicae.";
RL   Braz. J. Med. Biol. Res. 29:1599-1602(1996).
CC   -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC       complex that catalyzes the key enzymatic reactions in nitrogen
CC       fixation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC   -!- COFACTOR:
CC       Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC       Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC       the iron protein (nitrogenase component 2).
CC   -!- MISCELLANEOUS: Ala-188 is present instead of the usual Ser that would
CC       serve as a ligand for the 8Fe-7S cluster in the oxidized state.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z54207; CAA90934.1; -; Genomic_DNA.
DR   AlphaFoldDB; P77875; -.
DR   SMR; P77875; -.
DR   GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR   InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR   Pfam; PF11844; DUF3364; 1.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01286; nifK; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Oxidoreductase.
FT   CHAIN           1..>356
FT                   /note="Nitrogenase molybdenum-iron protein beta chain"
FT                   /id="PRO_0000153100"
FT   BINDING         70
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         95
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         153
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   NON_TER         356
SQ   SEQUENCE   356 AA;  39280 MW;  009F5D4037732530 CRC64;
     MPQNADSVLD HENLFRQPEY RELFARKKEE FEFAVPLDKV QDVAEWTKTK EYQQKNFARE
     ALTINPAKAC QPLGAVYAAV GFQNTLPFVH GSQGCVAYYR SHFSRHFKEP TSCVSSSMTE
     DAAVFGGLNN MIDGLANALS LYKPEMIAVS TTCMAEVIGD DLDSFIKNAK DKGSVPAEYD
     VPFAHTPAFV GSHVTGYDNA LKGILTHFWD GKAGTVPALE RKPNERINFI GGFDGFVVGN
     LPEVKRIFKL MGVDGTIVCD PSEVWNTPTD GQFRMYSGGT TKQEVIEALD AKATVVFQEF
     STLKTAKYIA EKGQEVVTLN HPMGVAGTDQ FLMELSRLSG QPIPAELELE RGRLVD