NIFK_NOSS1
ID NIFK_NOSS1 Reviewed; 512 AA.
AC P00468;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase;
DE AltName: Full=Nitrogenase component I;
GN Name=nifK; OrderedLocusNames=all1440;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16593247; DOI=10.1073/pnas.79.22.6782;
RA Mazur B.J., Chui C.-F.;
RT "Sequence of the gene coding for the beta-subunit of dinitrogenase from the
RT blue-green alga Anabaena.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:6782-6786(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR EMBL; J01539; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BA000019; BAB73397.1; -; Genomic_DNA.
DR PIR; A00546; NIAIMB.
DR PIR; AE1986; AE1986.
DR RefSeq; WP_010995612.1; NZ_RSCN01000040.1.
DR AlphaFoldDB; P00468; -.
DR SMR; P00468; -.
DR STRING; 103690.17130787; -.
DR EnsemblBacteria; BAB73397; BAB73397; BAB73397.
DR KEGG; ana:all1440; -.
DR eggNOG; COG2710; Bacteria.
DR OMA; GEYEMYP; -.
DR OrthoDB; 397330at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01286; nifK; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW Nucleotide-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..512
FT /note="Nitrogenase molybdenum-iron protein beta chain"
FT /id="PRO_0000153085"
FT BINDING 70
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 95
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 188
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT CONFLICT 271
FT /note="G -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 512 AA; 57545 MW; 002FEEB5DF816462 CRC64;
MPQNPERTVD HVDLFKQPEY TELFENKRKN FEGAHPPEEV ERVSEWTKSW DYREKNFARE
ALTVNPAKGC QPVGAMFAAL GFEGTLPFVQ GSQGCVAYFR THLSRHYKEP CSAVSSSMTE
DAAVFGGLNN MIEGMQVSYQ LYKPKMIAVC TTCMAEVIGD DLGAFITNSK NAGSIPQDFP
VPFAHTPSFV GSHITGYDNM MKGILSNLTE GKKKATSNGK INFIPGFDTY VGNNRELKRM
MGVMGVDYTI LSDSSDYFDS PNMGEYEMYP GGTKLEDAAD SINAKATVAL QAYTTPKTRE
YIKTQWKQET QVLRPFGVKG TDEFLTAVSE LTGKAIPEEL EIERGRLVDA ITDSYAWIHG
KKFAIYGDPD LIISITSFLL EMGAEPVHIL CNNGDDTFKK EMEAILAASP FGKEAKVWIQ
KDLWHFRSLL FTEPVDFFIG NSYGKYLWRD TSIPMVRIGY PLFDRHHLHR YSTLGYQGGL
NILNWVVNTL LDEMDRSTNI TGKTDISFDL IR
