NIFK_RIPO1
ID NIFK_RIPO1 Reviewed; 511 AA.
AC Q55030; B7JWY4;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase;
DE AltName: Full=Nitrogenase component I;
GN Name=nifK; OrderedLocusNames=PCC8801_1787;
OS Rippkaea orientalis (strain PCC 8801) (Cyanothece sp. (strain PCC 8801)).
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC Aphanothecaceae; Rippkaea; Rippkaea orientalis.
OX NCBI_TaxID=41431;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Chen H.M.;
RT "Synechococcus RF-1 nifHDK sequences.";
RL Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 8801;
RX PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA Sherman L.A., Pakrasi H.B.;
RT "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT unicellular nitrogen-fixing Cyanobacteria.";
RL MBio 2:E214-E214(2011).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR EMBL; U22146; AAA64845.1; -; Genomic_DNA.
DR EMBL; CP001287; ACK65833.1; -; Genomic_DNA.
DR RefSeq; WP_012595106.1; NC_011726.1.
DR AlphaFoldDB; Q55030; -.
DR SMR; Q55030; -.
DR STRING; 41431.PCC8801_1787; -.
DR PRIDE; Q55030; -.
DR EnsemblBacteria; ACK65833; ACK65833; PCC8801_1787.
DR KEGG; cyp:PCC8801_1787; -.
DR eggNOG; COG2710; Bacteria.
DR HOGENOM; CLU_025876_2_0_3; -.
DR OMA; GLNNMID; -.
DR OrthoDB; 397330at2; -.
DR Proteomes; UP000008204; Chromosome.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01286; nifK; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW Nucleotide-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..511
FT /note="Nitrogenase molybdenum-iron protein beta chain"
FT /id="PRO_0000153109"
FT BINDING 69
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 94
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT BINDING 187
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with alpha chain"
FT /evidence="ECO:0000250"
FT CONFLICT 4
FT /note="N -> K (in Ref. 1; AAA64845)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="Q -> K (in Ref. 1; AAA64845)"
FT /evidence="ECO:0000305"
FT CONFLICT 421
FT /note="D -> Y (in Ref. 1; AAA64845)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 511 AA; 56973 MW; 027FD36526082603 CRC64;
MSQNIDKIQD HVELFHQPEY QELFENKKAL QGMASDEKVA EIAEWTKTWE YREKNFAREA
LTINPAKACQ PLGAILAAVG FEGTLPFVHG SQGCVAYFRT HFTRHFKEPF SGVSSSMTED
AAVFGGLKNM IEGLQNAYSL YQPKMIAVCT TCMAEVIGDD LGSFIGNAKA DGSVPQDFPV
PFAHTPSFVG SHITGYDNMM KAILLNLTDG KKPTTSNGKV NFIPGFETYV GNLRELKHLT
SAMGVDATIL GDNELYLDSP NDGEFKMYQG GTTLEEGADA INATKTIALQ TYPTVKTLEY
IEKEWQQPTA TYRPWGIKGT DEFVMALSEL TGNPVPPELE LERGRAVDAM TDSHAWLHGK
KAAIYGDPDL VMGMLQFMLE MGVEPVHVLV HNSTTEFEEE AKALLASSPY GQKATVWGGK
DLWHLRSLLF TEPVDFLIGN SYGKYLWRDT KIPLIRIGYP IFDRHHLHRY STIGYNGAIN
LLNWIVNGLF EEIDRNTNIP SKTDISFDLV R
