ID   NIFL_KLEOX              Reviewed;         495 AA.
AC   P56267;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2003, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Nitrogen fixation regulatory protein;
GN   Name=nifL;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NG13;
RX   PubMed=3027503; DOI=10.1007/bf00430436;
RA   Kim Y.-M., Ahn K.-J., Beppu T., Uozumi T.;
RT   "Nucleotide sequence of the nifLA operon of Klebsiella oxytoca NG13 and
RT   characterization of the gene products.";
RL   Mol. Gen. Genet. 205:253-259(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=2838726; DOI=10.1111/j.1365-2958.1987.tb00524.x;
RA   Drummond M.H., Wootton J.C.;
RT   "Sequence of nifL from Klebsiella pneumoniae: mode of action and
RT   relationship to two families of regulatory proteins.";
RL   Mol. Microbiol. 1:37-44(1987).
RN   [3]
RP   MUTAGENESIS OF CYS-184 AND CYS-187.
RX   PubMed=1879702; DOI=10.1016/0378-1119(91)90395-r;
RA   Contraras A., Drummond M.H.;
RT   "Cys184 and Cys187 of NifL protein of Klebsiella pneumoniae are not
RT   absolutely required for inhibition of NifA activity.";
RL   Gene 103:83-86(1991).
RN   [4]
RP   MUTAGENESIS.
RX   PubMed=8481091; DOI=10.1007/bf00248484;
RA   Sidoti C., Harwood G., Ackerman R., Coppard J., Merrick M.J.;
RT   "Characterisation of mutations in the Klebsiella pneumoniae nitrogen
RT   fixation regulatory gene nifL which impair oxygen regulation.";
RL   Arch. Microbiol. 159:276-281(1993).
CC   -!- FUNCTION: Required for the inhibition of NifA activity in response to
CC       oxygen and low level of fixed nitrogen. {ECO:0000250}.
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DR   EMBL; D00339; BAA00244.1; -; Genomic_DNA.
DR   EMBL; Y00525; CAA68581.1; -; Genomic_DNA.
DR   EMBL; S59063; AAB26403.1; -; Genomic_DNA.
DR   RefSeq; WP_004138774.1; NZ_CABGNY010000020.1.
DR   AlphaFoldDB; P56267; -.
DR   STRING; 571.MC52_26860; -.
DR   eggNOG; COG2205; Bacteria.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00130; PAS; 1.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR014285; N_fixation_neg-reg_NifL.
DR   InterPro; IPR001610; PAC.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR000700; PAS-assoc_C.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013767; PAS_fold.
DR   Pfam; PF00989; PAS; 1.
DR   SMART; SM00086; PAC; 1.
DR   SMART; SM00091; PAS; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   TIGRFAMs; TIGR02938; nifL_nitrog; 1.
DR   TIGRFAMs; TIGR00229; sensory_box; 1.
DR   PROSITE; PS50113; PAC; 1.
DR   PROSITE; PS50112; PAS; 1.
PE   1: Evidence at protein level;
KW   Nitrogen fixation; Repeat; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..495
FT                   /note="Nitrogen fixation regulatory protein"
FT                   /id="PRO_0000074816"
FT   DOMAIN          23..93
FT                   /note="PAS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   DOMAIN          94..148
FT                   /note="PAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT   DOMAIN          151..174
FT                   /note="PAS 2; truncated"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   VARIANT         250
FT                   /note="M -> V (in strain: NG13)"
FT   VARIANT         425
FT                   /note="L -> F (in strain: NG13)"
FT   VARIANT         465
FT                   /note="R -> S (in strain: NG13)"
FT   MUTAGEN         184
FT                   /note="C->A: Almost no change in activity."
FT                   /evidence="ECO:0000269|PubMed:1879702"
FT   MUTAGEN         187
FT                   /note="C->A: Almost no change in activity."
FT                   /evidence="ECO:0000269|PubMed:1879702"
SQ   SEQUENCE   495 AA;  55310 MW;  CBA9BF201F08E309 CRC64;
     MTLNMMLDNA VPEAIAGALT QQHPGLFFTM VEQASVAISL TDARANIIYA NPAFCRQTGY
     SLAQLLNQNP RLLASSQTPR EIYQEMWQTL LQRQPWRGQL INQRRDGGLY LVDIDITPVL
     NPQGELEHYL AMQRDISVSY TLEQRLRNHM TLMEAVLNNI PAAVVVVDEQ DRVVMDNLAY
     KTFCADCGGK ELLVELQVSP RKMGPGAEQI LPVVVRGAVR WLSVTCWALP GVSEEASRYF
     VDSAPARTLM VIADCTQQRQ QQEQGRLDRL KQQMTAGKLL AAIRESLDAA LIQLNCPINM
     LAAARRLNGE GSGNVALDAA WREGEEAMAR LQRCRPSLEL ESNAVWPLQP FFDDLYALYR
     TRFDDRARLQ VDMASPHLVG FGQRTQLLAC LSLWLDRTLA LAAELPSVPL EIELYAEEDE
     GWLSLYLNDN VPLLQVRYAH SPDALNSPGK GMELRLIQTL VAYHRGAIEL ASRPQGGTSL
     VLRFPLFNTL TGGEQ