ID   NIFL_KLEPN              Reviewed;         495 AA.
AC   P06772;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Nitrogen fixation regulatory protein;
GN   Name=nifL;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3062178; DOI=10.1016/0022-2836(88)90205-7;
RA   Arnold W., Rump A., Klipp W., Priefer U.B., Puehler A.;
RT   "Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire
RT   nitrogen fixation gene cluster of Klebsiella pneumoniae.";
RL   J. Mol. Biol. 203:715-738(1988).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=10350621; DOI=10.1016/s0167-4838(99)00075-8;
RA   Schmitz R.A.;
RT   "NifL of Klebsiella pneumoniae: redox characterization in relation to the
RT   nitrogen source.";
RL   Biochim. Biophys. Acta 1431:462-470(1999).
RN   [3]
RP   FAD-BINDING.
RX   PubMed=9435114; DOI=10.1111/j.1574-6968.1997.tb12791.x;
RA   Schmitz R.A.;
RT   "NifL of Klebsiella pneumoniae carries an N-terminally bound FAD cofactor,
RT   which is not directly required for the inhibitory function of NifL.";
RL   FEMS Microbiol. Lett. 157:313-318(1997).
CC   -!- FUNCTION: Required for the inhibition of NifA activity in response to
CC       oxygen and low level of fixed nitrogen.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000269|PubMed:9435114};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Redox potential:
CC         E(0) is -277 mV.;
CC   -!- INTERACTION:
CC       P06772; Q9ZA65: glnK; NbExp=4; IntAct=EBI-8500088, EBI-8499900;
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DR   EMBL; X13303; CAA31681.1; -; Genomic_DNA.
DR   PIR; A48370; A48370.
DR   AlphaFoldDB; P06772; -.
DR   IntAct; P06772; 1.
DR   MINT; P06772; -.
DR   GO; GO:0009399; P:nitrogen fixation; IDA:CACAO.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00130; PAS; 1.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR014285; N_fixation_neg-reg_NifL.
DR   InterPro; IPR001610; PAC.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR000700; PAS-assoc_C.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013767; PAS_fold.
DR   Pfam; PF00989; PAS; 1.
DR   SMART; SM00086; PAC; 1.
DR   SMART; SM00091; PAS; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   TIGRFAMs; TIGR02938; nifL_nitrog; 1.
DR   TIGRFAMs; TIGR00229; sensory_box; 1.
DR   PROSITE; PS50113; PAC; 1.
DR   PROSITE; PS50112; PAS; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Nitrogen fixation; Repeat; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..495
FT                   /note="Nitrogen fixation regulatory protein"
FT                   /id="PRO_0000074817"
FT   DOMAIN          23..93
FT                   /note="PAS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   DOMAIN          94..148
FT                   /note="PAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT   DOMAIN          151..174
FT                   /note="PAS 2; truncated"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
SQ   SEQUENCE   495 AA;  55213 MW;  127DF145BB1C1957 CRC64;
     MTLNMMLDNA VPEAIAGALT QQHPGLFFTM VEQASVAISL TDARANITYA NPAFCRQTGY
     SLAQLLNQNP RLLASSQTPR EIYQEMWQTL LQRQPWRGQL INQARDGGLY LVDIDITPVL
     NPQGELEHYL AMQRDISVSY TLEQRLRNHM TLMEAVLNNI PAAVVVVDEQ DRVVMDNLAY
     KTFCADCGGK ELLVELQVSP RKMGPGAEQI LPVVVRGAVR WLSVTCWALP GVSEEASRYF
     VDSAPARTLM VIADCTQQRQ QQEQGRLDRL KQQMTAGKLL AAIRESLDAA LIQLNCPINM
     LAAARRLNGE GSGNVALDAA WREGEEAMAR LQRCRPSLEL ESNAVWPLQP FFDDLYALYR
     TRFDDRARLQ VDMASPHLVG FGQRTQLLAC LSLWLDRTLA LAAELPSVPL EIELYAEEDE
     GWLSLYLNDN VPLLQVRYAH SPDALNSPGK GMELRLIQTL VAYHRGAIEL ASRPQGGTSL
     VLRFPLFNTL TGGEQ