ID   NIFN_BRADU              Reviewed;         469 AA.
AC   P26507;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN;
GN   Name=nifN; OrderedLocusNames=blr1746;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=2266945; DOI=10.1007/bf00262436;
RA   Aguilar O.M., Taormino J., Thoeny B., Ramseier T., Hennecke H.,
RA   Szalay A.A.;
RT   "The nifEN genes participating in FeMo cofactor biosynthesis and genes
RT   encoding dinitrogenase are part of the same operon in Bradyrhizobium
RT   species.";
RL   Mol. Gen. Genet. 224:413-420(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=11157954; DOI=10.1128/jb.183.4.1405-1412.2001;
RA   Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R.,
RA   Hennecke H.;
RT   "Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA
RT   region of the Bradyrhizobium japonicum chromosome.";
RL   J. Bacteriol. 183:1405-1412(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC       prosthetic group of nitrogenase (FeMo cofactor).
CC   -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; X56894; CAA40214.1; -; Genomic_DNA.
DR   EMBL; AF322012; AAG60732.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47011.1; -; Genomic_DNA.
DR   PIR; S12264; NIZJMN.
DR   RefSeq; NP_768386.1; NC_004463.1.
DR   RefSeq; WP_011084555.1; NZ_CP011360.1.
DR   AlphaFoldDB; P26507; -.
DR   SMR; P26507; -.
DR   STRING; 224911.27349999; -.
DR   EnsemblBacteria; BAC47011; BAC47011; BAC47011.
DR   GeneID; 64067053; -.
DR   KEGG; bja:blr1746; -.
DR   PATRIC; fig|224911.44.peg.1211; -.
DR   eggNOG; COG2710; Bacteria.
DR   HOGENOM; CLU_025876_2_0_5; -.
DR   OMA; HFYFGGK; -.
DR   PhylomeDB; P26507; -.
DR   UniPathway; UPA00782; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   CDD; cd01966; Nitrogenase_NifN_1; 1.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR005975; Nase_Mo-Fe_CF.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01285; nifN; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nitrogen fixation; Reference proteome.
FT   CHAIN           1..469
FT                   /note="Nitrogenase iron-molybdenum cofactor biosynthesis
FT                   protein NifN"
FT                   /id="PRO_0000153127"
FT   REGION          435..469
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         44
FT                   /ligand="[7Fe-Mo-9S-C-homocitryl] cluster"
FT                   /ligand_id="ChEBI:CHEBI:30409"
FT                   /ligand_note="cofactor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   469 AA;  51232 MW;  7A735B3CA239B342 CRC64;
     MALVTAPTKA CVVNPLKMSQ PIGGAYAFMG LRGAMPLLHG SQGCTSFGLT LFVRHFKEAI
     PLQTTAMSEV ATVLGGYENL EQAILNISKR AKPKIIGICS TGVTETNGDD VEAYLKLIRS
     AYPQLTKLPL VYVSTPDFKG AFQDGWEKAV ARMVEVLVDR PSANGLRDPS KVNVLPGCHL
     TPGDLDELRA LLEDFGLYPS FLPDLAGSLD GHIPDEFTST TIGGIDVDEI ASMGRAGWTI
     AIGAQMQRAA EVMQTKTGVP FRVFERLCGL HPNDDFMMFL SEISGRPIPS KYRRQRSQLA
     DAMLDAHFHI GGRKVAIGAE PDLLFDLSGM LHDMGAQVTV AVTTTQSEVI ERIRTKEVLI
     GDLEDLEGFA KEKHCDLLIT HSHGRQAAGR LKVPFYRVGF PIFDRLGAGH QVSVGYRGTR
     NVIFQIANLV IAHRDENDRP TPDRWRTTPG LPQHVGHRRS TGAPERSIA