ID   NIFN_RHIME              Reviewed;         441 AA.
AC   P12781;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN;
GN   Name=nifN; Synonyms=fixF; OrderedLocusNames=RA0478; ORFNames=SMa0873;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=3316182; DOI=10.1128/jb.169.12.5393-5400.1987;
RA   Aguilar O.M., Reilaender H., Arnold W., Puehler A.;
RT   "Rhizobium meliloti nifN (fixF) gene is part of an operon regulated by a
RT   nifA-dependent promoter and codes for a polypeptide homologous to the nifK
RT   gene product.";
RL   J. Bacteriol. 169:5393-5400(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC       prosthetic group of nitrogenase (FeMo cofactor).
CC   -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; M18272; AAA88520.1; -; Genomic_DNA.
DR   EMBL; AE006469; AAK65136.1; -; Genomic_DNA.
DR   PIR; C28379; C28379.
DR   PIR; F95321; F95321.
DR   RefSeq; NP_435724.1; NC_003037.1.
DR   RefSeq; WP_010967458.1; NC_003037.1.
DR   AlphaFoldDB; P12781; -.
DR   SMR; P12781; -.
DR   EnsemblBacteria; AAK65136; AAK65136; SMa0873.
DR   GeneID; 61599279; -.
DR   KEGG; sme:SMa0873; -.
DR   PATRIC; fig|266834.11.peg.488; -.
DR   HOGENOM; CLU_025876_2_0_5; -.
DR   OMA; HFYFGGK; -.
DR   UniPathway; UPA00782; -.
DR   Proteomes; UP000001976; Plasmid pSymA.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   CDD; cd01966; Nitrogenase_NifN_1; 1.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR005975; Nase_Mo-Fe_CF.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01285; nifN; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nitrogen fixation; Plasmid; Reference proteome.
FT   CHAIN           1..441
FT                   /note="Nitrogenase iron-molybdenum cofactor biosynthesis
FT                   protein NifN"
FT                   /id="PRO_0000153131"
FT   BINDING         44
FT                   /ligand="[7Fe-Mo-9S-C-homocitryl] cluster"
FT                   /ligand_id="ChEBI:CHEBI:30409"
FT                   /ligand_note="cofactor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   441 AA;  47327 MW;  AFF54BD7B20402AB CRC64;
     MVRILSQTKW ATINPLKSSQ PLGGALAFLG VGGAIPLFHG SQGCTSFALV LLVRHFKEAI
     PLQTTAMDDV AIVLGGAGHL EQAILNLKIR AKPKLIGICT TALVETRGED LAGDLASIKL
     ERAEELTGTD VVLANTPDFD GAMEEGWAKA VTAMIKAITR IGEQERQSRT IAILPGWNLT
     IADIEQLRDI VESFGLKPII LPDLSGSLDG IVPDDRWVPT TYGGISVEEI RELGTAAQCI
     AIGEHMRGPA EEMKTLTGVP YVLFQSLTGL NAVDRFVSLL SSISGRPAPA KVRRRRAQLQ
     DALLDGHFHS AGKKIAIAAE PDQLYQLATF FICLGAEIVA AVTTKGASKI LHKVPVEIIQ
     VGDLGDLESL ATHADLLVTH SHGQHASARL GTPLMRVGFP VFDQLGSQHK LTILYHGTRD
     LIFEVSNIFQ SHSLAPTHRG T