ID   NIFSL_ASFM2             Reviewed;         376 AA.
AC   Q65236;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=NifS-like protein;
GN   OrderedLocusNames=Mal-132; ORFNames=j11R;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8021596; DOI=10.1099/0022-1317-75-7-1655;
RA   Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C.,
RA   Hammond J.M., Smith G.L.;
RT   "Nucleotide sequence of a 55 kbp region from the right end of the genome of
RT   a pathogenic African swine fever virus isolate (Malawi LIL20/1).";
RL   J. Gen. Virol. 75:1655-1684(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:P0A6B9};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:Q65192}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. NifS/IscS subfamily. {ECO:0000305}.
CC   -!- CAUTION: Although related to the NifS/IscS subfamily, lacks the
CC       conserved active site, suggesting it has no transferase activity.
CC       {ECO:0000305}.
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DR   EMBL; X71982; CAA50830.1; -; Genomic_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q65236; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   3: Inferred from homology;
KW   Virion.
FT   CHAIN           1..376
FT                   /note="NifS-like protein"
FT                   /id="PRO_0000373145"
FT   BINDING         58..59
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250|UniProtKB:P0A6B9"
FT   BINDING         184..186
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250|UniProtKB:P0A6B9"
SQ   SEQUENCE   376 AA;  41805 MW;  AB7D93399BC252BF CRC64;
     MASILALDGL YAEVPKFLPE ALREGCAGKK PLSFYIQQIL NLMGCDGDEY HVLFTSSSEE
     ANTHMITAAV RRHLLRTRQR PHVIIGAAEP PSVTECVKAL AREKRCVYTI IPLKNFEIDP
     VAVYDAIQSN TCLACISGTN AVVKTFNKLQ DISNVLRGIP LHSEVSDLVY QGCIQQNPPA
     DSFSLNSLYG FLGVGVLGMK KKVMQDLGPL IFGGGLRGGS PNIPGIHAMY RTLIQQRPSI
     KKINTIHKLF IKTLKKHQHV YLPGLSIEDV PSNGMPVEVP KGLPGYILFS VGHSAEELQK
     KIFTKFNVKV GRIVNLQEML FRIKIPQKYW ETLLFIQLRD VLTKEDIKRV MVVLMYLDTI
     TPRGSLPPPS HSSSFS