NIFU1_ORYSJ
ID NIFU1_ORYSJ Reviewed; 226 AA.
AC Q84LK7; Q0IPQ2;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=NifU-like protein 1, chloroplastic;
DE AltName: Full=OsNifu1;
DE Flags: Precursor;
GN Name=NIFU1; OrderedLocusNames=Os12g0176200, LOC_Os12g07700;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Shoot;
RA Asayama M.;
RT "Nuclear-encoded plastid gene, NifU1, in rice.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP PURIFICATION OF RECOMBINANT PROTEIN.
RX PubMed=15978837; DOI=10.1016/j.pep.2005.04.019;
RA Katoh S., Murata K., Kubota Y., Kumeta H., Ogura K., Inagaki F.,
RA Asayama M., Katoh E.;
RT "Refolding and purification of recombinant OsNifU1A domain II that was
RT expressed by Escherichia coli.";
RL Protein Expr. Purif. 43:149-156(2005).
RN [7]
RP STRUCTURE BY NMR OF 154-226.
RA Kumeta H., Ogura K., Asayama M., Katoh S., Katoh E., Inagaki F.;
RT "Solution structure of C-terminal domain of nifU-like protein from Oryza
RT sativa.";
RL Submitted (JUL-2005) to the PDB data bank.
CC -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of
CC chloroplastic iron-sulfur proteins. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR EMBL; AB096013; BAC76603.1; -; mRNA.
DR EMBL; DP000011; ABA96603.1; -; Genomic_DNA.
DR EMBL; AP008218; BAF29313.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT16109.1; -; Genomic_DNA.
DR RefSeq; XP_015619438.1; XM_015763952.1.
DR PDB; 1TH5; NMR; -; A=154-226.
DR PDB; 2JNV; NMR; -; A=73-153.
DR PDBsum; 1TH5; -.
DR PDBsum; 2JNV; -.
DR AlphaFoldDB; Q84LK7; -.
DR SMR; Q84LK7; -.
DR STRING; 4530.OS12T0176200-01; -.
DR PaxDb; Q84LK7; -.
DR PRIDE; Q84LK7; -.
DR EnsemblPlants; Os12t0176200-01; Os12t0176200-01; Os12g0176200.
DR GeneID; 4351651; -.
DR Gramene; Os12t0176200-01; Os12t0176200-01; Os12g0176200.
DR KEGG; osa:4351651; -.
DR eggNOG; KOG2358; Eukaryota.
DR HOGENOM; CLU_080894_2_0_1; -.
DR InParanoid; Q84LK7; -.
DR OMA; VAAPNHQ; -.
DR OrthoDB; 1536051at2759; -.
DR EvolutionaryTrace; Q84LK7; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q84LK7; OS.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IBA:GO_Central.
DR GO; GO:0005198; F:structural molecule activity; IEA:UniProt.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IBA:GO_Central.
DR Gene3D; 3.30.300.130; -; 2.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR Pfam; PF01106; NifU; 2.
DR SUPFAM; SSF117916; SSF117916; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Disulfide bond; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..76
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 77..226
FT /note="NifU-like protein 1, chloroplastic"
FT /id="PRO_0000247614"
FT DISULFID 116
FT /note="Interchain (with C-119)"
FT /evidence="ECO:0000250"
FT DISULFID 119
FT /note="Interchain (with C-116)"
FT /evidence="ECO:0000250"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:2JNV"
FT HELIX 79..84
FT /evidence="ECO:0007829|PDB:2JNV"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:2JNV"
FT HELIX 88..91
FT /evidence="ECO:0007829|PDB:2JNV"
FT TURN 92..95
FT /evidence="ECO:0007829|PDB:2JNV"
FT STRAND 98..103
FT /evidence="ECO:0007829|PDB:2JNV"
FT STRAND 105..112
FT /evidence="ECO:0007829|PDB:2JNV"
FT STRAND 116..119
FT /evidence="ECO:0007829|PDB:2JNV"
FT HELIX 122..135
FT /evidence="ECO:0007829|PDB:2JNV"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:2JNV"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:2JNV"
FT HELIX 158..165
FT /evidence="ECO:0007829|PDB:1TH5"
FT TURN 166..168
FT /evidence="ECO:0007829|PDB:1TH5"
FT HELIX 169..172
FT /evidence="ECO:0007829|PDB:1TH5"
FT TURN 173..176
FT /evidence="ECO:0007829|PDB:1TH5"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:1TH5"
FT STRAND 189..192
FT /evidence="ECO:0007829|PDB:1TH5"
FT STRAND 196..203
FT /evidence="ECO:0007829|PDB:1TH5"
FT HELIX 204..215
FT /evidence="ECO:0007829|PDB:1TH5"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:1TH5"
SQ SEQUENCE 226 AA; 23653 MW; ADD9AB2D2D540DD5 CRC64;
MQTTTVPMAA AAAVAPSTTT SSSASFKVAA YAWSSCRSSS SPATRLVAAP NHQRPPLVVG
AIAGLDPVTA VQLPLTAGNV ESVLDQVRPY LTADGGDVAL HEIAGNVVRL KLQGACGSCP
SSLITIKRGI ERRLMEKIPD VAAVEPVTDK ETGLELNEEN VEKVLNEIRP YLAGTGGGGL
QFLMIKGPIV KVRLTGPAAV VRTVRIAVSK KLREKIPSIQ IVQLLS
