ID   NIFU_AZOBR              Reviewed;         310 AA.
AC   Q43909; P70726;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Nitrogen fixation protein NifU;
GN   Name=nifU;
OS   Azospirillum brasilense.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Frazzon J.S., Schrank I.S.;
RT   "The nifU gene from Azospirillum brasilense.";
RL   Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9503607; DOI=10.1111/j.1574-6968.1998.tb12854.x;
RA   Frazzon J.S., Schrank I.S.;
RT   "Sequencing and complementation analysis of the nifUSV genes from
RT   Azospirillum brasilense.";
RL   FEMS Microbiol. Lett. 159:151-158(1998).
CC   -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC       present in iron-sulfur proteins. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; L34354; AAA22184.1; -; Genomic_DNA.
DR   EMBL; U26427; AAC46176.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q43909; -.
DR   SMR; Q43909; -.
DR   PRIDE; Q43909; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd06664; IscU_like; 1.
DR   Gene3D; 1.10.10.1100; -; 1.
DR   Gene3D; 3.30.300.130; -; 1.
DR   InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR   InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR   InterPro; IPR034904; FSCA_dom_sf.
DR   InterPro; IPR016217; N_fixation_NifU.
DR   InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR   InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF04324; Fer2_BFD; 1.
DR   Pfam; PF01106; NifU; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   PIRSF; PIRSF000375; NifU; 1.
DR   SUPFAM; SSF117916; SSF117916; 1.
DR   TIGRFAMs; TIGR02000; NifU_proper; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation.
FT   CHAIN           1..310
FT                   /note="Nitrogen fixation protein NifU"
FT                   /id="PRO_0000166166"
FT   REGION          195..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        56
FT                   /note="N -> K (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        133..136
FT                   /note="EEGE -> AAGT (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        141..144
FT                   /note="CFGI -> DFQAF (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149..152
FT                   /note="IERA -> DRRR (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        201..202
FT                   /note="Missing (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        212..223
FT                   /note="TPSSRWPRRPRH -> IAYQAAGPKAEA (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        230..231
FT                   /note="NV -> TC (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        235..236
FT                   /note="QK -> RR (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243..244
FT                   /note="DW -> EL (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        281..282
FT                   /note="GT -> AS (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        303..307
FT                   /note="ASLLP -> LSA (in Ref. 1; AAA22184)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   310 AA;  33201 MW;  EF9F474F91255FEF CRC64;
     MWNYTDKVKE HFFNPKNAGA LDEADDVGEV GSITCGDALK LMMKVNPDQP GHRDANFQTF
     GCGSAIASLR SALTEMIIGK TVDEALGQVT NRDIAEYLGG LPPEKMDCSV MGAEALPAAI
     ANFKGEGLVD DHEEGELVCK CFGIDAAMIE RAVTVNSLTT LEEVTHYTKA GGSCQTCHEK
     IEEVLEAVLA KTGAVGPAQA PSPTPPARSG WTPSSRWPRR PRHAPAKLTN VQRMQKIMFA
     IEDWRPQIQR DGGDVELVDI DGKDIYVRLT GACSGCSQSA GTMMGVQAKL VEALGEFVRV
     KPASLLPVGA