ID   NIFU_AZOVI              Reviewed;         312 AA.
AC   P05340;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Nitrogen fixation protein NifU;
GN   Name=nifU;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   PubMed=2644218; DOI=10.1128/jb.171.2.1017-1027.1989;
RA   Jacobson M.R., Brigle K.E., Bennett L.T., Setterquist R.A., Wilson M.S.,
RA   Cash V.L., Beynon J., Newton W.E., Dean D.R.;
RT   "Physical and genetic map of the major nif gene cluster from Azotobacter
RT   vinelandii.";
RL   J. Bacteriol. 171:1017-1027(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3040672; DOI=10.1128/jb.169.9.4024-4029.1987;
RA   Beynon J., Ally A., Cannon M., Cannon F., Jacobson M.R., Cash V.L.,
RA   Dean D.R.;
RT   "Comparative organization of nitrogen fixation-specific genes from
RT   Azotobacter vinelandii and Klebsiella pneumoniae: DNA sequence of the
RT   nifUSV genes.";
RL   J. Bacteriol. 169:4024-4029(1987).
RN   [3]
RP   EPR SPECTROSCOPY OF IRON-SULFUR CLUSTERS.
RX   PubMed=7947754; DOI=10.1021/bi00249a034;
RA   Fu W., Jack R.F., Morgan T.V., Dean D.R., Johnson M.K.;
RT   "nifU gene product from Azotobacter vinelandii is a homodimer that contains
RT   two identical 2Fe-2S clusters.";
RL   Biochemistry 33:13455-13463(1994).
RN   [4]
RP   EPR SPECTROSCOPY OF IRON-SULFUR CLUSTERS.
RX   PubMed=10819462; DOI=10.1007/s007750050361;
RA   Agar J.N., Yuvaniyama P., Jack R.F., Cash V.L., Smith A.D., Dean D.R.,
RA   Johnson M.K.;
RT   "Modular organization and identification of a mononuclear iron-binding site
RT   within the NifU protein.";
RL   J. Biol. Inorg. Chem. 5:167-177(2000).
CC   -!- FUNCTION: Involved in the formation or repair of [Fe-S] clusters
CC       present in iron-sulfur proteins.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC       Note=Binds 1 Fe cation per subunit.;
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. The redox potential of the
CC       [2Fe-2S] cluster is -254 mv.;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; M20568; AAA64725.1; -; Genomic_DNA.
DR   EMBL; M17349; AAA22167.1; -; Genomic_DNA.
DR   PIR; S29756; S29756.
DR   RefSeq; WP_012698853.1; NZ_FPKM01000020.1.
DR   AlphaFoldDB; P05340; -.
DR   SMR; P05340; -.
DR   OMA; IRPMLMM; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd06664; IscU_like; 1.
DR   Gene3D; 1.10.10.1100; -; 1.
DR   Gene3D; 3.30.300.130; -; 1.
DR   InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR   InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR   InterPro; IPR034904; FSCA_dom_sf.
DR   InterPro; IPR016217; N_fixation_NifU.
DR   InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR   InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF04324; Fer2_BFD; 1.
DR   Pfam; PF01106; NifU; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   PIRSF; PIRSF000375; NifU; 1.
DR   SUPFAM; SSF117916; SSF117916; 1.
DR   TIGRFAMs; TIGR02000; NifU_proper; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation.
FT   CHAIN           1..312
FT                   /note="Nitrogen fixation protein NifU"
FT                   /id="PRO_0000166168"
FT   BINDING         35
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT   BINDING         62
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT   BINDING         106
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT   BINDING         137
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT   BINDING         139
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT   BINDING         172
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT   BINDING         175
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT   CONFLICT        92
FT                   /note="I -> M (in Ref. 2; AAA22167)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        185
FT                   /note="E -> Q (in Ref. 2; AAA22167)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   312 AA;  33272 MW;  2C0F15473B402397 CRC64;
     MWDYSEKVKE HFYNPKNAGA VEGANAIGDV GSLSCGDALR LTLKVDPETD VILDAGFQTF
     GCGSAIASSS ALTEMVKGLT LDEALKISNQ DIADYLDGLP PEKMHCSVMG REALQAAVAN
     YRGETIEDDH EEGALICKCF AVDEVMVRDT IRANKLSTVE DVTNYTKAGG GCSACHEAIE
     RVLTEELAAR GEVFVAAPIK AKKKVKVLAP EPAPAPVAEA PAAAPKLSNL QRIRRIETVL
     AAIRPTLQRD KGDVELIDVD GKNVYVKLTG ACTGCQMASM TLGGIQQRLI EELGEFVKVI
     PVSAAAHAQM EV