ID   NIFV1_NOSS1             Reviewed;         377 AA.
AC   Q44290;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Homocitrate synthase 1;
DE            EC=2.3.3.14;
GN   Name=nifV1; Synonyms=nifV; OrderedLocusNames=alr1407;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9139910; DOI=10.1128/jb.179.9.2930-2937.1997;
RA   Stricker O., Masepohl B., Klipp W., Boehme H.;
RT   "Identification and characterization of the nifV-nifZ-nifT gene region from
RT   the filamentous cyanobacterium Anabaena sp. strain PCC 7120.";
RL   J. Bacteriol. 179:2930-2937(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- FUNCTION: This protein is a Fe-Mo-cofactor biosynthetic component.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA +
CC         H(+); Xref=Rhea:RHEA:12929, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:58884; EC=2.3.3.14;
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA68174.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X99902; CAA68174.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; BA000019; BAB73364.1; -; Genomic_DNA.
DR   PIR; AD1982; AD1982.
DR   RefSeq; WP_010995579.1; NZ_RSCN01000040.1.
DR   AlphaFoldDB; Q44290; -.
DR   SMR; Q44290; -.
DR   STRING; 103690.17130754; -.
DR   EnsemblBacteria; BAB73364; BAB73364; BAB73364.
DR   KEGG; ana:alr1407; -.
DR   eggNOG; COG0119; Bacteria.
DR   OMA; SNMFAHE; -.
DR   OrthoDB; 840579at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0004410; F:homocitrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   CDD; cd07939; DRE_TIM_NifV; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR013477; NifV/FrbC.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF00682; HMGL-like; 1.
DR   TIGRFAMs; TIGR02660; nifV_homocitr; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation; Reference proteome; Transferase.
FT   CHAIN           1..377
FT                   /note="Homocitrate synthase 1"
FT                   /id="PRO_0000140456"
FT   DOMAIN          4..255
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
FT   CONFLICT        99..103
FT                   /note="GIQIA -> DVK (in Ref. 1; CAA68174)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        203
FT                   /note="A -> L (in Ref. 1; CAA68174)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        349
FT                   /note="Q -> L (in Ref. 1; CAA68174)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   377 AA;  40851 MW;  7FB0C6706875AA0A CRC64;
     MNKVLINDTT LRDGEQAAGV VFTLEEKVAI AKFLDTIGVP ELEVGIPAMG EEEMRAICAI
     SNLGLKANLL AWNRAVISDI KASVACGMER VHIAIPVSGI QIAAKFHGQW RVSLQRLKDC
     ISFAVDQGLW VAVGGEDSSR ADENFLLDVA LYAQEWGASR FRFCDTVGVL DPFTTYGKVK
     LLVSALTIPV EVHTHNDFGM ATANALAGIK AGASSVNTTV IGLGERAGNA ALEEVVMAIK
     RIYGVDMGID TPRLLELSQL VAAASGANVP PWKAIVGENT FAHESGIHAH GVLQNPDTYE
     PFAPEEVGWE RRLVVGKHSG RHSVSNLLEQ HGIFLNPEET QSVLDAVRQQ SIKKKRSLTT
     EELLNLVKEQ RYSHAAR