ID   NIFVO_CLOPA             Reviewed;         352 AA.
AC   Q00852; Q6J2L5;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Homocitrate synthase, omega subunit;
DE            EC=2.3.3.14;
GN   Name=nifV-OMEGA;
OS   Clostridium pasteurianum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2022611; DOI=10.1128/jb.173.10.3041-3046.1991;
RA   Wang S.-Z., Dean D.R., Chen J.-S., Johnson J.L.;
RT   "The N-terminal and C-terminal portions of NifV are encoded by two
RT   different genes in Clostridium pasteurianum.";
RL   J. Bacteriol. 173:3041-3046(1991).
CC   -!- FUNCTION: This protein is a Fe-Mo-cofactor biosynthetic component.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA +
CC         H(+); Xref=Rhea:RHEA:12929, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:58884; EC=2.3.3.14;
CC   -!- SUBUNIT: Heterodimer of an alpha and an omega chain.
CC   -!- MISCELLANEOUS: In Clostridium pasteurianum the N-terminal and C-
CC       terminal portions of NifV are encoded by two different genes, nifV-
CC       alpha and nifV-omega.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000305}.
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DR   EMBL; AY603957; AAT37649.1; -; Genomic_DNA.
DR   PIR; A39403; A39403.
DR   AlphaFoldDB; Q00852; -.
DR   SMR; Q00852; -.
DR   GO; GO:0004410; F:homocitrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Nitrogen fixation; Transferase.
FT   CHAIN           1..352
FT                   /note="Homocitrate synthase, omega subunit"
FT                   /id="PRO_0000140468"
SQ   SEQUENCE   352 AA;  41578 MW;  4C6B0E6BBD484B1B CRC64;
     MAVVMDGVKK NIIDRTIPNL VKKFQNFNND DIAYFLKLLH ETGIDLFEIN RDSMDKIKKF
     PLNLDYIYRI ENIEDYNYLN NYNFKYIILN YKTIYRFLLE DEKIQKNLKE HNIILEIDIE
     DLDELYLSED NKIFCIFNIV CLRINNLSKL DFIDEDFRIK DLKSKFNVLV DFCASNKYNM
     ATAIIINAFL NGSDIITTEF NSNDYAAMEE VIIALKSIRN IEIRGDLKLI SKLTRIYEKI
     TSERVYSMKP ILGEDIFKYE SGIHADGIAK NPKNYEPFNP ELIGTNRKLY IGKHSGKAAL
     VVKFKELNLN CNNIDMNLFL QDIREKSIQE KRNVLDNEII EMYKEYNKSY QR