ID   NIFV_AZOBR              Reviewed;         376 AA.
AC   P70728;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Homocitrate synthase;
DE            EC=2.3.3.14;
GN   Name=nifV;
OS   Azospirillum brasilense.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29145 / DSM 1690 / IMET 11303 / Sp7;
RX   PubMed=9503607; DOI=10.1111/j.1574-6968.1998.tb12854.x;
RA   Frazzon J.S., Schrank I.S.;
RT   "Sequencing and complementation analysis of the nifUSV genes from
RT   Azospirillum brasilense.";
RL   FEMS Microbiol. Lett. 159:151-158(1998).
CC   -!- FUNCTION: This protein is a Fe-Mo-cofactor biosynthetic component.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA +
CC         H(+); Xref=Rhea:RHEA:12929, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:58884; EC=2.3.3.14;
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000305}.
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DR   EMBL; U26427; AAC46178.1; -; Genomic_DNA.
DR   AlphaFoldDB; P70728; -.
DR   SMR; P70728; -.
DR   GO; GO:0004410; F:homocitrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF00682; HMGL-like; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation; Transferase.
FT   CHAIN           1..376
FT                   /note="Homocitrate synthase"
FT                   /id="PRO_0000140458"
FT   DOMAIN          5..252
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ   SEQUENCE   376 AA;  39701 MW;  B1FD476EBD220C8D CRC64;
     MAGSTIINDT HARDGEQTAG VAFTLDEKIA IAQALDEAGV AELEIGIPAM GREERERIRA
     VASLGLKARL MVWCRMHDTD LKARSTAVAP SLSVPVSDIH ITKKLNGSRA WALREIERKV
     KTARDHGLEV SLGGEDSSRA DMDFLIAAAT VAQRPRFRFA DTLGVLDPFQ HARLHLTAAA
     TDPEIGYHAH TPRLANATAW VTLAAVLGGA THVNTTVNGL GERAGNAPLE EVVVSLKVLY
     GQDCGVDTRA LGAISDLVER ASNRPVAVNK SIVRDAVFTH EAGIHVDGLL TDRAPTRIST
     PPRWGASTAS CWASIPARGG EDGLRQLGIA CDDATAQACC RGSGRATRAK PPDGGGAARP
     FHDDACWQAF PAAGGV