NIFV_ENTAG
ID NIFV_ENTAG Reviewed; 400 AA.
AC Q52070;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Homocitrate synthase;
DE EC=2.3.3.14;
GN Name=nifV;
OS Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OG Plasmid pEA3.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX NCBI_TaxID=549;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lechler A.;
RL Thesis (1993), University of Bayreuth, Germany.
CC -!- FUNCTION: This protein is a Fe-Mo-cofactor biosynthetic component.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA +
CC H(+); Xref=Rhea:RHEA:12929, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:58884; EC=2.3.3.14;
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. {ECO:0000305}.
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DR EMBL; X99694; CAA68021.1; -; Genomic_DNA.
DR AlphaFoldDB; Q52070; -.
DR SMR; Q52070; -.
DR GO; GO:0004410; F:homocitrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR013477; NifV/FrbC.
DR InterPro; IPR000891; PYR_CT.
DR Pfam; PF00682; HMGL-like; 1.
DR TIGRFAMs; TIGR02660; nifV_homocitr; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Nitrogen fixation; Plasmid; Transferase.
FT CHAIN 1..400
FT /note="Homocitrate synthase"
FT /id="PRO_0000140461"
FT DOMAIN 18..269
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ SEQUENCE 400 AA; 43441 MW; AAEDA3BA73A26D1D CRC64;
MAASESERTG GGNRPMAVII NDTTLRDGEQ SPGVAFRASE KLEIAQALVN AGVRELEAGT
PALGEEECVR LAILRRQLPD TVMMSWCRMN SAEIHDSARV GMDWVDISVP ASDLLREQKL
HRPWPAVIAS LKPLVQLAHR LGMRVCMGCE DASRSADETL HMLAEVAADL GIERMRFADT
LGILDPFSTF TRISALRQHW HGGLEIHAHN DLGMATANTL AAVRAGATHV NTTVLGLGER
AGNAALESVT LSLDRCLHLD CGIDFTELPA LCRLVAAAAG RTIDVQHPLV GGQVFTHESG
LHVAALLRDP RSYQGIDPSL VGREFNLVLG KHSGRQAVSG ICARLGYILS ETQTLLMLQE
VKRFAEHFKR NPGEDEVIAM CQMRMDDQIP FEGQLQAQGG