ID   NIG2A_ODOGR             Reviewed;          66 AA.
AC   P85071; P0C2A5;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Nigrocin-2GRa {ECO:0000303|PubMed:16621155};
DE   AltName: Full=Grahamin-1 {ECO:0000303|PubMed:16487561};
DE   Flags: Precursor;
OS   Odorrana grahami (Yunnanfu frog) (Rana grahami).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Odorrana.
OX   NCBI_TaxID=167935 {ECO:0000303|PubMed:16487561};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 46-66, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16487561};
RX   PubMed=16487561; DOI=10.1016/j.toxicon.2006.01.002;
RA   Xu X., Li J., Han Y., Yang H., Liang J., Lu Q., Lai R.;
RT   "Two antimicrobial peptides from skin secretions of Rana grahami.";
RL   Toxicon 47:459-464(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 46-66, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000303|PubMed:16621155};
RX   PubMed=16621155; DOI=10.1016/j.peptides.2006.03.002;
RA   Conlon J.M., Al-Ghaferi N., Abraham B., Jiansheng H., Cosette P.,
RA   Leprince J., Jouenne T., Vaudry H.;
RT   "Antimicrobial peptides from diverse families isolated from the skin of the
RT   Asian frog, Rana grahami.";
RL   Peptides 27:2111-2117(2006).
CC   -!- FUNCTION: Antimicrobial peptide active at least against the Gram-
CC       positive bacterium S.aureus but with otherwise unclear activity
CC       spectrum (PubMed:16487561, PubMed:16621155). Lacks hemolytic activity
CC       against rabbit or human erythrocytes (PubMed:16487561,
CC       PubMed:16621155). {ECO:0000269|PubMed:16487561,
CC       ECO:0000269|PubMed:16621155}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16487561}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:16487561}.
CC   -!- MASS SPECTROMETRY: Mass=1965; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:16487561};
CC   -!- MASS SPECTROMETRY: Mass=1964.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16621155};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P85071; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR032749; Nigrocin.
DR   Pfam; PF16047; Antimicrobial22; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..45
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000273289"
FT   PEPTIDE         46..66
FT                   /note="Nigrocin-2GRa"
FT                   /evidence="ECO:0000269|PubMed:16487561,
FT                   ECO:0000269|PubMed:16621155"
FT                   /id="PRO_0000273290"
FT   DISULFID        60..66
FT                   /evidence="ECO:0000250|UniProtKB:P39084"
SQ   SEQUENCE   66 AA;  7282 MW;  F34AD177EB5A78CF CRC64;
     MFTLKKSQLL LFFPGTINLS LCQDETNAEE ERRDEEVAKM EEIKRGLLSG ILGAGKHIVC
     GLSGLC