NIG2A_ODOJI
ID NIG2A_ODOJI Reviewed; 21 AA.
AC B3A0M6;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 12.
DE RecName: Full=Nigrocin-2JDa {ECO:0000303|PubMed:22917879};
OS Odorrana jingdongensis (Jingdong frog) (Rana jingdongensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Odorrana.
OX NCBI_TaxID=431936;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC TISSUE=Skin secretion {ECO:0000269|PubMed:22917879};
RX PubMed=22917879; DOI=10.1016/j.jprot.2012.08.004;
RA Liu J., Jiang J., Wu Z., Xie F.;
RT "Antimicrobial peptides from the skin of the Asian frog, Odorrana
RT jingdongensis: De novo sequencing and analysis of tandem mass spectrometry
RT data.";
RL J. Proteomics 75:5807-5821(2012).
CC -!- FUNCTION: Has antibacterial activity against E.coli ATCC 25992 (MIC=16
CC uM), E.coli CIB 84492 (MIC=16 uM), S.aureus ATCC 25923 (MIC=16 uM) and
CC S.aureus CIB 85462 (MIC=8 uM). Has antifungal activity against
CC C.albicans (MIC=63 uM). Has hemolytic activity against rabbit
CC erythrocytes. {ECO:0000269|PubMed:22917879}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:22917879}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000305|PubMed:22917879}.
CC -!- MASS SPECTROMETRY: Mass=2032.1465; Mass_error=0.0009;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:22917879};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Brevinin subfamily. {ECO:0000255}.
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DR AlphaFoldDB; B3A0M6; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
DR InterPro; IPR032749; Nigrocin.
DR Pfam; PF16047; Antimicrobial22; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW Direct protein sequencing; Disulfide bond; Fungicide; Hemolysis; Secreted.
FT PEPTIDE 1..21
FT /note="Nigrocin-2JDa"
FT /evidence="ECO:0000269|PubMed:22917879"
FT /id="PRO_0000420137"
FT DISULFID 15..21
FT /evidence="ECO:0000269|PubMed:22917879"
FT UNSURE 14
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:22917879"
FT UNSURE 17
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:22917879"
FT UNSURE 20
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:22917879"
SQ SEQUENCE 21 AA; 2035 MW; 068869BBF356EB91 CRC64;
GIFGKILGAG KKVLCGLSGL C