ID   NIGR1_PELNI             Reviewed;          33 AA.
AC   P0C008;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Nigrocin-1;
OS   Pelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX   NCBI_TaxID=8409;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Skin secretion;
RX   PubMed=11682065; DOI=10.1016/s0014-5793(01)02956-8;
RA   Park S., Park S.-H., Ahn H.-C., Kim S., Kim S.S., Lee B.J., Lee B.-J.;
RT   "Structural study of novel antimicrobial peptides, nigrocins, isolated from
RT   Rana nigromaculata.";
RL   FEBS Lett. 507:95-100(2001).
CC   -!- FUNCTION: Shows antibacterial activity against both Gram-positive and
CC       Gram-negative bacteria and against the fungus C.albicans. Has no
CC       hemolytic activity.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C008; -.
DR   SMR; P0C008; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..33
FT                   /note="Nigrocin-1"
FT                   /id="PRO_0000044738"
FT   DISULFID        27..33
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   33 AA;  3348 MW;  77D0AC540791EC1D CRC64;
     GLLDSIKGMA ISAGKGALQN LLKVASCKLD KTC