ID   NIGR2_PELNI             Reviewed;          21 AA.
AC   P0C009;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Nigrocin-2;
OS   Pelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX   NCBI_TaxID=8409;
RN   [1]
RP   PROTEIN SEQUENCE, AND CIRCULAR DICHROISM ANALYSIS.
RC   TISSUE=Skin secretion;
RX   PubMed=11682065; DOI=10.1016/s0014-5793(01)02956-8;
RA   Park S., Park S.-H., Ahn H.-C., Kim S., Kim S.S., Lee B.J., Lee B.-J.;
RT   "Structural study of novel antimicrobial peptides, nigrocins, isolated from
RT   Rana nigromaculata.";
RL   FEBS Lett. 507:95-100(2001).
CC   -!- FUNCTION: Thanks to its single linear amphipathic alpha-helix, may
CC       integrate into membrane phospholipids, leading to lysis of the
CC       membrane. Shows antibacterial activity against both Gram-positive and
CC       Gram-negative bacteria and against the fungus C.albicans. Has no
CC       hemolytic activity.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0C009; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IEA:UniProt.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR032749; Nigrocin.
DR   Pfam; PF16047; Antimicrobial22; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..21
FT                   /note="Nigrocin-2"
FT                   /id="PRO_0000043814"
FT   DISULFID        15..21
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   21 AA;  2031 MW;  EA42BE10228DEB89 CRC64;
     GLLSKVLGVG KKVLCGVSGL C