NIKB_STAA8
ID NIKB_STAA8 Reviewed; 328 AA.
AC Q2FYQ5; Q84AN7; Q9ZGN6;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Nickel import system permease protein NikB {ECO:0000305};
GN Name=nikB {ECO:0000303|PubMed:20662775}; Synonyms=opp-2B, oppB2;
GN OrderedLocusNames=SAOUHSC_01380;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9791183; DOI=10.1046/j.1365-2958.1998.01075.x;
RA Coulter S.N., Schwan W.R., Ng E.Y.W., Langhorne M.H., Ritchie H.D.,
RA Westbrock-Wadman S., Hufnagle W.O., Folger K.R., Bayer A.S., Stover C.K.;
RT "Staphylococcus aureus genetic loci impacting growth and survival in
RT multiple infection environments.";
RL Mol. Microbiol. 30:393-404(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Cramton S.E., Madimidou F., Goetz F.;
RT "Role of the oligopeptide permease (opp-2) operon in Staphylococcus aureus
RT biofilm formation.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [4]
RP FUNCTION, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=RN6390;
RX PubMed=20662775; DOI=10.1111/j.1365-2958.2010.07287.x;
RA Hiron A., Posteraro B., Carriere M., Remy L., Delporte C., La Sorda M.,
RA Sanguinetti M., Juillard V., Borezee-Durant E.;
RT "A nickel ABC-transporter of Staphylococcus aureus is involved in urinary
RT tract infection.";
RL Mol. Microbiol. 77:1246-1260(2010).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE (Opp2) involved
CC in nickel import. Probably responsible for the translocation of the
CC substrate across the membrane. Required for full urease activity and
CC plays a significant role in the virulence of S.aureus during urinary
CC tract infection (UTI). {ECO:0000269|PubMed:20662775}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000269|PubMed:20662775}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Transcription is stable during the exponential phase and
CC increases only in standard conditions in late exponential phase.
CC {ECO:0000269|PubMed:20662775}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the nikBCDE operon strongly reduces
CC nickel transport and urease activity. Mutant shows decreased virulence
CC in a mouse model of ascending UTI. {ECO:0000269|PubMed:20662775}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. OppBC subfamily. {ECO:0000305}.
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DR EMBL; AF076684; AAC69843.1; -; Genomic_DNA.
DR EMBL; AY205146; AAO47755.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD30475.1; -; Genomic_DNA.
DR RefSeq; WP_000469949.1; NZ_LS483365.1.
DR RefSeq; YP_499907.1; NC_007795.1.
DR AlphaFoldDB; Q2FYQ5; -.
DR SMR; Q2FYQ5; -.
DR STRING; 1280.SAXN108_1397; -.
DR EnsemblBacteria; ABD30475; ABD30475; SAOUHSC_01380.
DR GeneID; 3920789; -.
DR KEGG; sao:SAOUHSC_01380; -.
DR PATRIC; fig|93061.5.peg.1264; -.
DR eggNOG; COG0601; Bacteria.
DR HOGENOM; CLU_036879_0_2_9; -.
DR OMA; WPGMGTF; -.
DR PRO; PR:Q2FYQ5; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR045621; BPD_transp_1_N.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR Pfam; PF19300; BPD_transp_1_N; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..328
FT /note="Nickel import system permease protein NikB"
FT /id="PRO_0000272185"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 100..297
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 328 AA; 36814 MW; DF28C17B1DB7D871 CRC64;
MFIIKSMLYR LMQMIVVLFV ISTLTFILMK LSPGNPVDKI LHLDVAQVST EQINATKDKL
GLNDSLLVQW WHWMNHLLHF NLGKSFESKE PVTQILFNYA PITLLISFST LVVSLCISIP
LGIIAAKRFH KWTDKVIRVI STLSISLPAF FIGIILLFIV TNLMNIDSVI LSQFILPVIT
LSLGMCAYII RLVRSNLLML LQSNIVQASR LRGMNERYIL IHDLLKPTIL PIIPLLGISL
GSLIGGTVVI ENLFDIPGIG YLLMDSIKSR DYPVIQGCVL FIGFFVVIIN TIADLLTLLL
DPKQRLQLGN PKIKTNTPLI SESSDRHA
