ID   NIKB_STAAR              Reviewed;         328 AA.
AC   Q6GH25;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Nickel import system permease protein NikB {ECO:0000250|UniProtKB:Q2FYQ5};
GN   Name=nikB {ECO:0000250|UniProtKB:Q2FYQ5}; Synonyms=oppB2;
GN   OrderedLocusNames=SAR1395;
OS   Staphylococcus aureus (strain MRSA252).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282458;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MRSA252;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex NikABCDE (Opp2) involved
CC       in nickel import. Probably responsible for the translocation of the
CC       substrate across the membrane. {ECO:0000250|UniProtKB:Q2FYQ5}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC       NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC       protein (NikA). {ECO:0000250|UniProtKB:Q2FYQ5}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. OppBC subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX571856; CAG40392.1; -; Genomic_DNA.
DR   RefSeq; WP_000469932.1; NC_002952.2.
DR   AlphaFoldDB; Q6GH25; -.
DR   SMR; Q6GH25; -.
DR   KEGG; sar:SAR1395; -.
DR   HOGENOM; CLU_036879_0_2_9; -.
DR   OMA; WPGMGTF; -.
DR   OrthoDB; 1479518at2; -.
DR   Proteomes; UP000000596; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR045621; BPD_transp_1_N.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   Pfam; PF19300; BPD_transp_1_N; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..328
FT                   /note="Nickel import system permease protein NikB"
FT                   /id="PRO_0000276776"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        229..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          100..297
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   328 AA;  36644 MW;  6F4EB3C12B8E04E5 CRC64;
     MFIIKSILYR LIQMIVVLFV ISTLAFILMK LSPGNPVDKI LHLDVAQVST EQINATKDKL
     GLNDSLLVQW WHWMNHLLHF NLGKSFESKE PVTQILFNYA PITLLISFST LVVSLCISIP
     LGIIAAKRFH KLSDKVIRVI STLSISLPAF FIGIILLFIV TNLMNIDSVI LSQFILPVLT
     LSLGMCAYII RLVRSNLLIL LKSNIVQASR LRGMNERYIL IHDLLKPTIL PIIPLLGISL
     GSLIGGTVVI ENLFDIPGIG YLLMDSIKSR DYPVIQGCVL FIGFFVVIIN TIADLLTLLL
     DPKQRLQLGN PKNKTNTPLI SESSDRHA