NIKC_ECOLI
ID NIKC_ECOLI Reviewed; 277 AA.
AC P0AFA9; P33592; Q2M7E1;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Nickel transport system permease protein NikC;
GN Name=nikC; OrderedLocusNames=b3478, JW3443;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7934931; DOI=10.1111/j.1365-2958.1993.tb01247.x;
RA Navarro C., Wu L.-F., Mandrand-Berthelot M.-A.;
RT "The nik operon of Escherichia coli encodes a periplasmic binding-protein-
RT dependent transport system for nickel.";
RL Mol. Microbiol. 9:1181-1191(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in a nickel transport system, probably translocates
CC nickel through the bacterial inner membrane.
CC -!- SUBUNIT: Probably forms a heterodimeric pore with NikB.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. OppBC subfamily. {ECO:0000305}.
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DR EMBL; X73143; CAA51661.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18453.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76503.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77815.1; -; Genomic_DNA.
DR PIR; S39596; S39596.
DR RefSeq; NP_417935.1; NC_000913.3.
DR RefSeq; WP_001008963.1; NZ_STEB01000004.1.
DR AlphaFoldDB; P0AFA9; -.
DR BioGRID; 4263255; 7.
DR ComplexPortal; CPX-4348; Nickel ABC transporter complex.
DR DIP; DIP-48065N; -.
DR STRING; 511145.b3478; -.
DR TCDB; 3.A.1.5.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P0AFA9; -.
DR PRIDE; P0AFA9; -.
DR EnsemblBacteria; AAC76503; AAC76503; b3478.
DR EnsemblBacteria; BAE77815; BAE77815; BAE77815.
DR GeneID; 67417108; -.
DR GeneID; 947990; -.
DR KEGG; ecj:JW3443; -.
DR KEGG; eco:b3478; -.
DR PATRIC; fig|1411691.4.peg.3247; -.
DR EchoBASE; EB2002; -.
DR eggNOG; COG1173; Bacteria.
DR HOGENOM; CLU_028518_1_1_6; -.
DR OMA; TDWFLVM; -.
DR PhylomeDB; P0AFA9; -.
DR BioCyc; EcoCyc:NIKC-MON; -.
DR BioCyc; MetaCyc:NIKC-MON; -.
DR PRO; PR:P0AFA9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016151; F:nickel cation binding; IDA:EcoCyc.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0098716; P:nickel cation import across plasma membrane; IC:ComplexPortal.
DR GO; GO:0035444; P:nickel cation transmembrane transport; IMP:EcoCyc.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR014157; Nickel_NikC.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR TIGRFAMs; TIGR02790; nickel_nikC; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion transport; Membrane; Nickel;
KW Nickel transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..277
FT /note="Nickel transport system permease protein NikC"
FT /id="PRO_0000060121"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 34..78
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 100..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 142..183
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 205..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 229..239
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 261..277
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 67..260
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 277 AA; 30362 MW; F64769E44EEA1D6F CRC64;
MNFFLSSRWS VRLALIIIAL LALIALTSQW WLPYDPQAID LPSRLLSPDA QHWLGTDHLG
RDIFSRLMAA TRVSLGSVMA CLLLVLTLGL VIGGSAGLIG GRVDQATMRV ADMFMTFPTS
ILSFFMVGVL GTGLTNVIIA IALSHWAWYA RMVRSLVISL RQREFVLASR LSGAGHVRVF
VDHLAGAVIP SLLVLATLDI GHMMLHVAGM SFLGLGVTAP TAEWGVMIND ARQYIWTQPL
QMFWPGLALF ISVMAFNLVG DALRDHLDPH LVTEHAH
