NIKC_STAA8
ID NIKC_STAA8 Reviewed; 276 AA.
AC Q2FYQ6; Q84AN6; Q9ZGN5;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Nickel import system permease protein NikC {ECO:0000305};
GN Name=nikC {ECO:0000303|PubMed:20662775}; Synonyms=opp-2C, oppC2;
GN OrderedLocusNames=SAOUHSC_01379;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX PubMed=9791183; DOI=10.1046/j.1365-2958.1998.01075.x;
RA Coulter S.N., Schwan W.R., Ng E.Y.W., Langhorne M.H., Ritchie H.D.,
RA Westbrock-Wadman S., Hufnagle W.O., Folger K.R., Bayer A.S., Stover C.K.;
RT "Staphylococcus aureus genetic loci impacting growth and survival in
RT multiple infection environments.";
RL Mol. Microbiol. 30:393-404(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Cramton S.E., Madimidou F., Goetz F.;
RT "Role of the oligopeptide permease (opp-2) operon in Staphylococcus aureus
RT biofilm formation.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [4]
RP FUNCTION, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=RN6390;
RX PubMed=20662775; DOI=10.1111/j.1365-2958.2010.07287.x;
RA Hiron A., Posteraro B., Carriere M., Remy L., Delporte C., La Sorda M.,
RA Sanguinetti M., Juillard V., Borezee-Durant E.;
RT "A nickel ABC-transporter of Staphylococcus aureus is involved in urinary
RT tract infection.";
RL Mol. Microbiol. 77:1246-1260(2010).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE (Opp2) involved
CC in nickel import. Probably responsible for the translocation of the
CC substrate across the membrane. Required for full urease activity and
CC plays a significant role in the virulence of S.aureus during urinary
CC tract infection (UTI). {ECO:0000269|PubMed:20662775}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000269|PubMed:20662775}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Transcription is stable during the exponential phase and
CC increases only in standard conditions in late exponential phase.
CC {ECO:0000269|PubMed:20662775}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the nikBCDE operon strongly reduces
CC nickel transport and urease activity. Mutant shows decreased virulence
CC in a mouse model of ascending UTI (PubMed:20662775). Insertion mutant
CC shows attenuated growth in several infection models (PubMed:9791183).
CC {ECO:0000269|PubMed:20662775, ECO:0000269|PubMed:9791183}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. OppBC subfamily. {ECO:0000305}.
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DR EMBL; AF076684; AAC69844.1; -; Genomic_DNA.
DR EMBL; AY205146; AAO47756.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD30474.1; -; Genomic_DNA.
DR RefSeq; WP_000548932.1; NZ_LS483365.1.
DR RefSeq; YP_499906.1; NC_007795.1.
DR AlphaFoldDB; Q2FYQ6; -.
DR STRING; 1280.SAXN108_1396; -.
DR EnsemblBacteria; ABD30474; ABD30474; SAOUHSC_01379.
DR GeneID; 3920788; -.
DR KEGG; sao:SAOUHSC_01379; -.
DR PATRIC; fig|93061.5.peg.1263; -.
DR eggNOG; COG1173; Bacteria.
DR HOGENOM; CLU_028518_5_3_9; -.
DR OMA; VPTYFRM; -.
DR PRO; PR:Q2FYQ6; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..276
FT /note="Nickel import system permease protein NikC"
FT /id="PRO_0000272186"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 69..258
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 276 AA; 31255 MW; 0DE81551833549A0 CRC64;
MHKIFSKNNL IFFVFVAFIF VVIVLQFFVS SENATKVNLS QTFEPISWLH LLGTDDYGRD
LFTRIIIGAR STLFVTVLTL IAIVVIGVTL GLFAGYKKGW IERLVLRFID VGLSIPEFII
MIALASFFQP SLWNLVISIT LIKWMNYTRL TRSIVNSEMN KPYIKMAQLF HVPTRTILIR
HLTPKIIPAI IVLMVVDFGK IILYISSLSF IGLGAQPPTP EWGAMLQQGR DFISSHPIML
IAPASVIAIT ILIFNLTGDA LRDRLLKQRG EYDESH
