ID   NIKE_BRUAB              Reviewed;         266 AA.
AC   Q578S7;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712};
DE            EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712};
GN   Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712}; OrderedLocusNames=BruAb2_0432;
OS   Brucella abortus biovar 1 (strain 9-941).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=262698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=9-941;
RX   PubMed=15805518; DOI=10.1128/jb.187.8.2715-2726.2005;
RA   Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z.,
RA   Li L.-L., Kapur V., Alt D.P., Olsen S.C.;
RT   "Completion of the genome sequence of Brucella abortus and comparison to
RT   the highly similar genomes of Brucella melitensis and Brucella suis.";
RL   J. Bacteriol. 187:2715-2726(2005).
CC   -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in
CC       nickel import. Responsible for energy coupling to the transport system.
CC       {ECO:0000255|HAMAP-Rule:MF_01712}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC         Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC         ChEBI:CHEBI:456216; EC=7.2.2.11; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01712};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC       NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC       protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01712}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel importer
CC       (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}.
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DR   EMBL; AE017224; AAX75857.1; -; Genomic_DNA.
DR   RefSeq; WP_002965842.1; NC_006933.1.
DR   AlphaFoldDB; Q578S7; -.
DR   SMR; Q578S7; -.
DR   EnsemblBacteria; AAX75857; AAX75857; BruAb2_0432.
DR   GeneID; 3827147; -.
DR   KEGG; bmb:BruAb2_0432; -.
DR   HOGENOM; CLU_000604_1_23_5; -.
DR   OMA; LVERFCH; -.
DR   Proteomes; UP000000540; Chromosome II.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR014137; Nickel_NikE.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02769; nickel_nikE; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51248; NIKE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Nickel; Nickel transport; Nucleotide-binding; Translocase; Transport.
FT   CHAIN           1..266
FT                   /note="Nickel import ATP-binding protein NikE"
FT                   /id="PRO_0000260203"
FT   DOMAIN          4..252
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT   BINDING         45..52
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
SQ   SEQUENCE   266 AA;  29180 MW;  CBC0D12AA2731DFB CRC64;
     MSLISADNIV KIYQSHSLVG ASARKTMLHD ISISIGQGET VALLGRSGCG KSTLARLLVG
     LERPTSGEVR FRGVPLTKLD RSGMKAFRRE VQLIFQDSPG AVNARSSVRA IIGEPLRHLT
     SLDETRREER IQELLRLVEL PPEIADRLPA QVSGGQLQRI CIARALAVNP KLIILDEAVS
     NLDIHLQASA LALLTKLQQE GGIAYLFVTH DLRLVQKFAA RCLVMDEGQI VEEIKTADLD
     SMRHPASRLL REAVLPPLPV RAVETN