NIKE_ECO57
ID NIKE_ECO57 Reviewed; 268 AA.
AC Q8X4L6; Q7AA44;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712};
DE EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712};
GN Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712};
GN OrderedLocusNames=Z4872, ECs4347;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in
CC nickel import. Responsible for energy coupling to the transport system.
CC {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC ChEBI:CHEBI:456216; EC=7.2.2.11; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01712};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01712}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel importer
CC (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}.
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DR EMBL; AE005174; AAG58607.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37770.1; -; Genomic_DNA.
DR PIR; C86018; C86018.
DR PIR; C91172; C91172.
DR RefSeq; NP_312374.1; NC_002695.1.
DR RefSeq; WP_000173697.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8X4L6; -.
DR SMR; Q8X4L6; -.
DR STRING; 155864.EDL933_4711; -.
DR EnsemblBacteria; AAG58607; AAG58607; Z4872.
DR EnsemblBacteria; BAB37770; BAB37770; ECs_4347.
DR GeneID; 915802; -.
DR KEGG; ece:Z4872; -.
DR KEGG; ecs:ECs_4347; -.
DR PATRIC; fig|386585.9.peg.4540; -.
DR eggNOG; COG1124; Bacteria.
DR HOGENOM; CLU_000604_1_23_6; -.
DR OMA; LVERFCH; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR014137; Nickel_NikE.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02769; nickel_nikE; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51248; NIKE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nickel; Nickel transport; Nucleotide-binding; Reference proteome;
KW Translocase; Transport.
FT CHAIN 1..268
FT /note="Nickel import ATP-binding protein NikE"
FT /id="PRO_0000092629"
FT DOMAIN 4..252
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
SQ SEQUENCE 268 AA; 29726 MW; B8F457452072F232 CRC64;
MTLLNVSDLS HHYAHGGFSG KHQHQAVLNN VSLTLKSGET VALLGRSGCG KSTLSRLLVG
LESPSQGNIS WRGESLAKLN RAQRKAFRRD IQMVFQDSIS AVNPRKTVRE ILREPMRHLL
SLKKSEQLAR ASEMLHAVDL DDSVLDKRPP QLSGGQLQRV CLARALAVEP KLLILDEAVS
NLDLVLQAGV IRLLKKLQQQ FGTACLFITH DLRLVERFCQ RVMVMDNGQI VETQAVGEKL
TFSSDAGRVL QNAILPAFPV RRRATEKV
