NIKE_ECOLI
ID NIKE_ECOLI Reviewed; 268 AA.
AC P33594; Q2M7E3;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712};
DE EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712};
GN Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712};
GN OrderedLocusNames=b3480, JW3445;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7934931; DOI=10.1111/j.1365-2958.1993.tb01247.x;
RA Navarro C., Wu L.-F., Mandrand-Berthelot M.-A.;
RT "The nik operon of Escherichia coli encodes a periplasmic binding-protein-
RT dependent transport system for nickel.";
RL Mol. Microbiol. 9:1181-1191(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in
CC nickel import. Responsible for energy coupling to the transport system.
CC {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC ChEBI:CHEBI:456216; EC=7.2.2.11; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01712};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01712}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel importer
CC (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}.
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DR EMBL; X73143; CAA51663.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18455.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76505.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77813.1; -; Genomic_DNA.
DR PIR; C65145; C65145.
DR RefSeq; NP_417937.1; NC_000913.3.
DR RefSeq; WP_000173631.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P33594; -.
DR SMR; P33594; -.
DR BioGRID; 4262508; 18.
DR BioGRID; 852296; 2.
DR ComplexPortal; CPX-4348; Nickel ABC transporter complex.
DR DIP; DIP-10344N; -.
DR IntAct; P33594; 11.
DR STRING; 511145.b3480; -.
DR TCDB; 3.A.1.5.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P33594; -.
DR PRIDE; P33594; -.
DR EnsemblBacteria; AAC76505; AAC76505; b3480.
DR EnsemblBacteria; BAE77813; BAE77813; BAE77813.
DR GeneID; 947987; -.
DR KEGG; ecj:JW3445; -.
DR KEGG; eco:b3480; -.
DR PATRIC; fig|1411691.4.peg.3245; -.
DR EchoBASE; EB2004; -.
DR eggNOG; COG1124; Bacteria.
DR HOGENOM; CLU_000604_1_23_6; -.
DR InParanoid; P33594; -.
DR OMA; LVERFCH; -.
DR PhylomeDB; P33594; -.
DR BioCyc; EcoCyc:NIKE-MON; -.
DR BioCyc; MetaCyc:NIKE-MON; -.
DR PRO; PR:P33594; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0098716; P:nickel cation import across plasma membrane; IC:ComplexPortal.
DR GO; GO:0035444; P:nickel cation transmembrane transport; IMP:EcoCyc.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR014137; Nickel_NikE.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02769; nickel_nikE; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51248; NIKE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nickel; Nickel transport; Nucleotide-binding; Reference proteome;
KW Translocase; Transport.
FT CHAIN 1..268
FT /note="Nickel import ATP-binding protein NikE"
FT /id="PRO_0000092627"
FT DOMAIN 4..252
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT CONFLICT 46..47
FT /note="RS -> GT (in Ref. 1; CAA51663)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="R -> A (in Ref. 1; CAA51663)"
FT /evidence="ECO:0000305"
FT CONFLICT 132..133
FT /note="SE -> RQ (in Ref. 1; CAA51663)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 268 AA; 29722 MW; 8413CB9E8D7C585A CRC64;
MTLLNISGLS HHYAHGGFNG KHQHQAVLNN VSLTLKSGET VALLGRSGCG KSTLARLLVG
LESPAQGNIS WRGEPLAKLN RAQRKAFRRD IQMVFQDSIS AVNPRKTVRE ILREPMRHLL
SLKKSEQLAR ASEMLKAVDL DDSVLDKRPP QLSGGQLQRV CLARALAVEP KLLILDEAVS
NLDLVLQAGV IRLLKKLQQQ FGTACLFITH DLRLVERFCQ RVMVMDNGQI VETQVVGEKL
TFSSDAGRVL QNAVLPAFPV RRRTTEKV
