NIKE_PSEPK
ID NIKE_PSEPK Reviewed; 273 AA.
AC Q88HL0;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712};
DE EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712};
GN Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712}; OrderedLocusNames=PP_3346;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in
CC nickel import. Responsible for energy coupling to the transport system.
CC {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC ChEBI:CHEBI:456216; EC=7.2.2.11; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01712};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01712}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel importer
CC (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}.
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DR EMBL; AE015451; AAN68950.1; -; Genomic_DNA.
DR RefSeq; NP_745486.1; NC_002947.4.
DR RefSeq; WP_010954222.1; NC_002947.4.
DR AlphaFoldDB; Q88HL0; -.
DR SMR; Q88HL0; -.
DR STRING; 160488.PP_3346; -.
DR EnsemblBacteria; AAN68950; AAN68950; PP_3346.
DR KEGG; ppu:PP_3346; -.
DR PATRIC; fig|160488.4.peg.3558; -.
DR eggNOG; COG1124; Bacteria.
DR HOGENOM; CLU_000604_1_23_6; -.
DR OMA; LVERFCH; -.
DR PhylomeDB; Q88HL0; -.
DR BioCyc; PPUT160488:G1G01-3579-MON; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR014137; Nickel_NikE.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02769; nickel_nikE; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51248; NIKE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nickel; Nickel transport; Nucleotide-binding; Reference proteome;
KW Translocase; Transport.
FT CHAIN 1..273
FT /note="Nickel import ATP-binding protein NikE"
FT /id="PRO_0000092630"
FT DOMAIN 13..252
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
SQ SEQUENCE 273 AA; 29974 MW; B78701DDF80DDD94 CRC64;
MSLLHVHHVG HRYRTGGLLR KRGWLQVLDG IDLQLHAGES IGLLGSSGSG KSTLARLLLG
LEKPAQGQVS FAGQDVSQLK GEQARAFQRT VQLVFQDAPG AFNPQRSIGW SIAEPLRHLT
DMDEAARQAR TLALLEEMGL RAEHAQRLPQ QLSGGQLQRA NIARALAASP QLVVLDEALS
NLDRLLQLQI LQQLEALRQR SGTAFVLISH DLSLVQYFCQ RVVLLHGGRI VEERPVTHDL
CFAHPVGRQL QAAVLPVRPQ RRPSPQGLPT AAH
