NIKE_SHIF8
ID NIKE_SHIF8 Reviewed; 268 AA.
AC Q0SZJ3;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712};
DE EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712};
GN Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712}; OrderedLocusNames=SFV_3483;
OS Shigella flexneri serotype 5b (strain 8401).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=373384;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=8401;
RX PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J.,
RA Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.;
RT "Complete genome sequence of Shigella flexneri 5b and comparison with
RT Shigella flexneri 2a.";
RL BMC Genomics 7:173-173(2006).
CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in
CC nickel import. Responsible for energy coupling to the transport system.
CC {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC ChEBI:CHEBI:456216; EC=7.2.2.11; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01712};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01712}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel importer
CC (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}.
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DR EMBL; CP000266; ABF05522.1; -; Genomic_DNA.
DR RefSeq; WP_000173670.1; NC_008258.1.
DR AlphaFoldDB; Q0SZJ3; -.
DR SMR; Q0SZJ3; -.
DR EnsemblBacteria; ABF05522; ABF05522; SFV_3483.
DR GeneID; 58388370; -.
DR KEGG; sfv:SFV_3483; -.
DR HOGENOM; CLU_000604_1_23_6; -.
DR OMA; LVERFCH; -.
DR BioCyc; SFLE373384:SFV_RS19150-MON; -.
DR Proteomes; UP000000659; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR014137; Nickel_NikE.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02769; nickel_nikE; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51248; NIKE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nickel; Nickel transport; Nucleotide-binding; Translocase; Transport.
FT CHAIN 1..268
FT /note="Nickel import ATP-binding protein NikE"
FT /id="PRO_0000274125"
FT DOMAIN 4..252
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01712"
SQ SEQUENCE 268 AA; 29575 MW; 5818D318012C43FF CRC64;
MTLLNVCGLS HHYAHGGFSG KHQHQAVLNN VSLTLKSGET VALLGRSGCG KSTLARLLVG
LESPSQGNIS WRGESLAKLN RAQRKAFRRD IQMVFQDSIS AVNPRKTVRE ILREPMRHLL
SLKKSEQLAR ASEMLKAVDL DDSVLDKRPP QLSGGQLQLV CLARALAVEP KLLILDEAVS
NLDLVLQAGV IRLLKKLQQQ FGTACLFITH DLRLVERFCQ RVMVMDNGQI AETQAVGDKL
TFSSDAGRVL QNAVLPAFPV RRRTSEKV