ID   NIKE_STAAM              Reviewed;         233 AA.
AC   Q99UA3;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Nickel import system ATP-binding protein NikE {ECO:0000250|UniProtKB:Q2FYQ8};
DE            EC=7.2.2.11 {ECO:0000250|UniProtKB:Q2FYQ8};
GN   Name=nikE {ECO:0000250|UniProtKB:Q2FYQ8}; Synonyms=oppF2;
GN   OrderedLocusNames=SAV1379;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Part of the ABC transporter complex NikABCDE (Opp2) involved
CC       in nickel import. Probably responsible for energy coupling to the
CC       transport system. {ECO:0000250|UniProtKB:Q2FYQ8}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC         Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC         ChEBI:CHEBI:456216; EC=7.2.2.11;
CC         Evidence={ECO:0000250|UniProtKB:Q2FYQ8};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15558;
CC         Evidence={ECO:0000250|UniProtKB:Q2FYQ8};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC       NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC       protein (NikA). {ECO:0000250|UniProtKB:Q2FYQ8}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; BA000017; BAB57541.1; -; Genomic_DNA.
DR   RefSeq; WP_000571259.1; NC_002758.2.
DR   AlphaFoldDB; Q99UA3; -.
DR   SMR; Q99UA3; -.
DR   PaxDb; Q99UA3; -.
DR   EnsemblBacteria; BAB57541; BAB57541; SAV1379.
DR   KEGG; sav:SAV1379; -.
DR   HOGENOM; CLU_000604_1_23_9; -.
DR   OMA; CDRTVHW; -.
DR   PhylomeDB; Q99UA3; -.
DR   BioCyc; SAUR158878:SAV_RS07425-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Ion transport; Membrane; Nickel;
KW   Nickel transport; Nucleotide-binding; Translocase; Transport.
FT   CHAIN           1..233
FT                   /note="Nickel import system ATP-binding protein NikE"
FT                   /id="PRO_0000276805"
FT   DOMAIN          2..228
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         35..42
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   233 AA;  26291 MW;  E367D75B86C92B0B CRC64;
     MIELKHVTFG YNKKQMVLQD INITIPDGEN VGILGESGCG KSTLASLVLG LFKPVKGEIY
     LSDNAVLTIF QHPLTSFNPD WTIETSLKEA LYYYRGLTDN TAQDQLLLQH LSTFELNAQL
     LTKLPSEVSG GQLQRFNVMR SLLAQPRVLI CDEITSNLDV IAEQNVINIL KAQTITNLNH
     FIVISHDLSV LQRLVNRIIV LKDGMIVDDF AIEELFNVDR HPYTKELVQT FSY