ID   NIKE_STAAR              Reviewed;         233 AA.
AC   Q6GH28;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Nickel import system ATP-binding protein NikE {ECO:0000250|UniProtKB:Q2FYQ8};
DE            EC=7.2.2.11 {ECO:0000250|UniProtKB:Q2FYQ8};
GN   Name=nikE {ECO:0000250|UniProtKB:Q2FYQ8}; Synonyms=oppF2;
GN   OrderedLocusNames=SAR1392;
OS   Staphylococcus aureus (strain MRSA252).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282458;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MRSA252;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex NikABCDE (Opp2) involved
CC       in nickel import. Probably responsible for energy coupling to the
CC       transport system. {ECO:0000250|UniProtKB:Q2FYQ8}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + phosphate;
CC         Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:49786,
CC         ChEBI:CHEBI:456216; EC=7.2.2.11;
CC         Evidence={ECO:0000250|UniProtKB:Q2FYQ8};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15558;
CC         Evidence={ECO:0000250|UniProtKB:Q2FYQ8};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD and
CC       NikE), two transmembrane proteins (NikB and NikC) and a solute-binding
CC       protein (NikA). {ECO:0000250|UniProtKB:Q2FYQ8}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; BX571856; CAG40389.1; -; Genomic_DNA.
DR   RefSeq; WP_000571253.1; NC_002952.2.
DR   AlphaFoldDB; Q6GH28; -.
DR   SMR; Q6GH28; -.
DR   KEGG; sar:SAR1392; -.
DR   HOGENOM; CLU_000604_1_23_9; -.
DR   OMA; CDRTVHW; -.
DR   OrthoDB; 1303270at2; -.
DR   Proteomes; UP000000596; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015413; F:ABC-type nickel transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Ion transport; Membrane; Nickel;
KW   Nickel transport; Nucleotide-binding; Translocase; Transport.
FT   CHAIN           1..233
FT                   /note="Nickel import system ATP-binding protein NikE"
FT                   /id="PRO_0000276803"
FT   DOMAIN          2..228
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         35..42
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   233 AA;  26287 MW;  36B38345C3DCFD77 CRC64;
     MIELKHVTFG YNKKQMVLQD INITIPDGEN VGILGESGCG KSTLASLVLG LFKPVKGEIY
     LSDNAVLPIF QHPLTSFNPD WTIETSLKEA LYYYRGLTDN TAQDQLLIQH LSTFELNAQL
     LTKLPSEVSG GQLQRFNVMR SLLAQPRVLI CDEITSNLDV IAEQNVINIL KAQTITNLNH
     FIVISHDLSV LQRLVNRIIV LKDGMIVDDF TIEELFNVDR HPYTKELVQA FSY