ID   NIKO_RHOCB              Reviewed;         254 AA.
AC   D5AQY6;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Nickel import ATP-binding protein NikO;
DE            EC=3.6.3.-;
DE   AltName: Full=Energy-coupling factor transporter ATP-binding protein NikO;
DE            Short=ECF transporter A component NikO;
GN   Name=nikO; Synonyms=cbiO2; OrderedLocusNames=RCAP_rcc01032;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
RN   [2]
RP   FUNCTION IN NICKEL TRANSPORT, SUBSTRATES, SUBUNIT, AND EXPRESSION IN
RP   E.COLI.
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=16352848; DOI=10.1128/jb.188.1.317-327.2006;
RA   Rodionov D.A., Hebbeln P., Gelfand M.S., Eitinger T.;
RT   "Comparative and functional genomic analysis of prokaryotic nickel and
RT   cobalt uptake transporters: evidence for a novel group of ATP-binding
RT   cassette transporters.";
RL   J. Bacteriol. 188:317-327(2006).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       NikMNQO involved in nickel import. The complex confers nickel uptake
CC       upon expression in E.coli; can also transport cobalt with a very low
CC       affinity. Presumably responsible for energy coupling to the transport
CC       system. {ECO:0000269|PubMed:16352848}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, NikO), a transmembrane
CC       protein (T component, NikQ) and a fused possible substrate-capture
CC       protein (S component, NikMN) of unknown stoichimetry. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; CP001312; ADE84792.1; -; Genomic_DNA.
DR   RefSeq; WP_013066771.1; NC_014034.1.
DR   AlphaFoldDB; D5AQY6; -.
DR   SMR; D5AQY6; -.
DR   STRING; 272942.RCAP_rcc01032; -.
DR   TCDB; 3.A.1.23.7; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; ADE84792; ADE84792; RCAP_rcc01032.
DR   GeneID; 31489959; -.
DR   KEGG; rcp:RCAP_rcc01032; -.
DR   eggNOG; COG1122; Bacteria.
DR   HOGENOM; CLU_000604_1_22_5; -.
DR   OMA; FVFQRPA; -.
DR   OrthoDB; 1752365at2; -.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015675; P:nickel cation transport; IDA:UniProtKB.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Hydrolase; Ion transport;
KW   Membrane; Nickel; Nickel transport; Nucleotide-binding; Reference proteome;
KW   Transport.
FT   CHAIN           1..254
FT                   /note="Nickel import ATP-binding protein NikO"
FT                   /id="PRO_0000411086"
FT   DOMAIN          5..246
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         37..44
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   254 AA;  27039 MW;  E1516853AD967B97 CRC64;
     MTPAFELQGV QFAYKGVPAL NGLDLTLPLG RRTALLGANG SGKSTLLRLL DGLQFPAAGR
     ISAFGTPLTE AMFTDEAAAI AFRRRVGFVF QNPEVQLFCP SVFDELAFGP LQLHWPKERI
     RARVARAIAQ FGLGPLAGRP PHRLSGGEKK RVALASVLIL DPEVLLLDEP TAALDPQATD
     DIAALLETEF GARNPGRTLI FSSHDLDLVA RIADHVVVLE AGKVAAAGPA AEVLARTALL
     RRARLLPGFD GTAP