ID   NIKR1_METAC             Reviewed;         140 AA.
AC   Q8TQZ1;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   21-NOV-2003, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Putative nickel-responsive regulator 1;
GN   OrderedLocusNames=MA_1395;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Transcriptional regulator. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC       Note=Binds 1 nickel ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM04811.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE010299; AAM04811.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_048065107.1; NC_003552.1.
DR   AlphaFoldDB; Q8TQZ1; -.
DR   SMR; Q8TQZ1; -.
DR   EnsemblBacteria; AAM04811; AAM04811; MA_1395.
DR   GeneID; 1473283; -.
DR   KEGG; mac:MA_1395; -.
DR   HOGENOM; CLU_113319_1_2_2; -.
DR   InParanoid; Q8TQZ1; -.
DR   OMA; NCLEVIH; -.
DR   OrthoDB; 90041at2157; -.
DR   PhylomeDB; Q8TQZ1; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 3.30.70.1150; -; 1.
DR   HAMAP; MF_00476; NikR; 1.
DR   InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR   InterPro; IPR045865; ACT-like_dom_sf.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR002145; CopG.
DR   InterPro; IPR022988; Ni_resp_reg_NikR.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR   Pfam; PF08753; NikR_C; 1.
DR   Pfam; PF01402; RHH_1; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF55021; SSF55021; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nickel; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..140
FT                   /note="Putative nickel-responsive regulator 1"
FT                   /id="PRO_0000139299"
FT   BINDING         81
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         92
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         94
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
FT   BINDING         100
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   140 AA;  15927 MW;  1FFA915D89222386 CRC64;
     METELMRIGI SLPDILLGEF DEIIGKRGYS SRSEGIRDAI RSYISYYEWM NDIKGHRVGT
     IAFVYDHTKR GLSNSLTDIQ HHYSHLIKSS IHVHLDHKEC FEVVVLNGDG KEIAELSETM
     MVLKGVKFSK LTTVASNENI